We have developed a method for fabricating combinatorial libraries of porous, three-dimensional, salt-leached, polymer scaffolds that can be used for screening the effect of scaffold properties on cell response. Syringe pumps, tubing and a static mixer are used to blend polymer solutions which are deposited in a combinatorial fashion as a single graded scaffold or as an array of discrete scaffolds in a 96-well plate. The method is inexpensive to employ requiring only syringe pumps and average lab supplies (polymers, syringes, tubing, solvent, etc.) for implementation. In addition, the approach is versatile since it is amenable to making combinatorial libraries with variations in scaffold composition, scaffold functional group (peptides, reactive groups, bioactive ligands) or scaffold nano-structure (nanoparticles, nanotopography).Both the gradient scaffold libraries and discrete scaffold arrays can serve as a platform to screen cell response to scaffold properties, while the gradient scaffold libraries might also serve as templates for generating functionally graded tissues.
, Stephens, J.
and Becker, M.
Combinatorial Polymer Scaffold Libraries, Advanced Materials, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852654
(Accessed November 30, 2023)