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Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells

Published

Author(s)

Subhadip Bodhak, Sheng Lin-Gibson, Carl G. Simon Jr., Luis Fernandez de Castro Diaz, Pamela G. Robey, Sergei A. Kuznetsov, Tina Kilts, Marian F. Young

Abstract

The use of in vivo transplantation assays under defined experimental conditions has become a valuable standard for evaluating osteogenic potential of different stem cell populations and is the subject of ongoing pre-clinical reconstructive therapy research. Therefore, development of a cost effective in vivo transplantation assay technique offering low volume sampling and rapid analysis in a high-throughput manner is of great interest due to the escalating health care costs. Herein, we have successfully validated an in vivo combinatorial transplant model by developing a miniature cassette transplant with multiple slots for screening osteogenesis of various human bone marrow stromal cells (hBMSCs) and bone grafting formulations (e.g., HA/TCP powder, Gelfoam) in subcutaneous space of immunodeficient mice. Our results confirmed that microwell-type combinatorial cassette transplant model can be used in in vivo cell based transplantation assay applications to efficiently, systematically and rapidly test as many as hBMSCs/vehicles combinations for maximizing osteogenesis.
Proceedings Title
Society for Biomaterials 2014 Annual Meeting & Exposition
Volume
1
Issue
1
Conference Dates
April 16-19, 2014
Conference Location
Denver, CO

Keywords

Combinatorial In Vivo Screening, Osteogenesis, Human Bone Marrow Stromal Cells, Mouse subcutaneous surgery, Histology

Citation

Bodhak, S. , Lin-Gibson, S. , Simon, C. , Fernandez, L. , Robey, P. , Kuznetsov, S. , Kilts, T. and Young, M. (2014), Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells, Society for Biomaterials 2014 Annual Meeting & Exposition, Denver, CO (Accessed June 30, 2022)
Created April 19, 2014, Updated February 19, 2017