Combinatorial Approach to Study Enzyme/Surface Interactions
K Loos, S L. Kennedy, Naomi Eidelman, Y Tai, M Zharnikov, Eric J. Amis, A Ulman, R A. Gross
A fast combinatorial approach to access information on the immobilization behavior and kinetics of enzymes on a variation of surfaces is presented. As a test system, Candida Antarctica Lipase B (CAL-B) was immobilized on a self-assembled monolayer (SAM) bearing a gradient of surface energy. The respective immobilization behavior was monitored by FTIR microspectroscopy. In addition, the activity of the immobilized enzyme was monitored over the entire film in real time with a specially developed fluorescence activity assay embedded into a siloxane gel. It was found that the highest amount of active protein was immobilized on the hydrophilic end of the gradient surface. This effect is presumably associated with a higher surface roughness of this area resulting in hydrophobic microenviroments in which the enzyme gets immobilized.
, Kennedy, S.
, Eidelman, N.
, Tai, Y.
, Zharnikov, M.
, Amis, E.
, Ulman, A.
and Gross, R.
Combinatorial Approach to Study Enzyme/Surface Interactions, Langmuir
(Accessed June 3, 2023)