Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Characterization of Laminin on Silanized Polydimethylsiloxane

Published

Author(s)

Joy P. Dunkers, Lisa M. Hughes, Hae-Jeong Lee, Marvi Matos, Marcus T. Cicerone

Abstract

In this work, we characterize a protein surface on a flexible polydimethylsiloxane substrate (PDMS) capable of undergoing strain. We compare the results from two different surface treatments: physically adsorbed laminin on PDMS and laminin deposited on silane treated PDMS. Immunofluorescence and x-ray reflectivity were used to measure the amount of laminin from each surface treatment. Vascular smooth muscle cell attachment and proliferation were compared between the methods. Additional work will concentrate on evaluating the robustness of surface treatments under strain.
Conference Dates
August 17-21, 2008
Conference Location
Philadelphia, PA
Conference Title
ACS Fall Meeting

Keywords

laminin, polydimethylsiloxane, smmoth muscle cells, X-ray reflectivity

Citation

Dunkers, J. , Hughes, L. , Lee, H. , Matos, M. and Cicerone, M. (2008), Characterization of Laminin on Silanized Polydimethylsiloxane, ACS Fall Meeting, Philadelphia, PA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=853602 (Accessed June 24, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created August 21, 2008, Updated February 19, 2017