Characterization of Laminin on Silanized Polydimethylsiloxane
Joy P. Dunkers, Lisa M. Hughes, Hae-Jeong Lee, Marvi Matos, Marcus T. Cicerone
In this work, we characterize a protein surface on a flexible polydimethylsiloxane substrate (PDMS) capable of undergoing strain. We compare the results from two different surface treatments: physically adsorbed laminin on PDMS and laminin deposited on silane treated PDMS. Immunofluorescence and x-ray reflectivity were used to measure the amount of laminin from each surface treatment. Vascular smooth muscle cell attachment and proliferation were compared between the methods. Additional work will concentrate on evaluating the robustness of surface treatments under strain.
, Hughes, L.
, Lee, H.
, Matos, M.
and Cicerone, M.
Characterization of Laminin on Silanized Polydimethylsiloxane, ACS Fall Meeting, Philadelphia, PA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=853602
(Accessed December 5, 2023)