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Certification of Drugs of Abuse in a Human Serum Standard Reference Material: SRM 1959



Susan S. Tai, Jocelyn L. Prendergast, Lorna T. Sniegoski, Michael J. Welch, Karen W. Phinney, Nien F. Zhang


A new Standard Reference Material (SRM) for drugs of abuse in human serum (SRM 1959) has been developed. SRM 1959 is a frozen human serum material fortified with seven drugs of abuse, benzoylecgonine (BZE), methadone (METH), methamphetamine (MAMP), morphine (MOR), nordiazepam (NOR), phencyclidine (PCP), and 11-nor-{Δ}9-tetrahydrocannabinol-9-carboxylic acid (THC-9-COOH). Two independent methods involving ID-GC/MS and ID-LC/MS were used for the value assignment. For THC- 9-COOH, an additional measurement using LC/MS/MS, was also included. All methods used isotopically labeled compounds as internal standards, and solid phase extractions to isolate the analytes from the serum. The GC/MS methods used different clean-up procedures from those used for the LC/MS-based methods. Repeatability with within-set coefficients of variation (CVs) ranged from 0.5 % to 4.3 % for the GC/MS methods and ranged from 0.2 % to 1.2 % for the LC/MS-based methods. Intermediate precision with between-set CVs for all the methods ranged from 0.1 % to 1.1 %. Agreement between the GC/MS and LC/MS methods ranged from 0.8 % to 8.8 %. The results from the methods were combined to obtain the certified concentrations and their expanded uncertainties. This SRM can be used as a control material for laboratories performing analysis of drugs of abuse in blood to test the accuracy of their methods.
Analytical and Bioanalytical Chemistry


Drugs of abuse, gas chromatography/mass spectrometry, GC/MS, isotope dilution, LC/MS, LC/MS/MS, liquid chromatography/mass spectrometry, liquid chromatography/tandem mass spectrometry, solid phase extraction, SRM, Standard Reference Material


Tai, S. , Prendergast, J. , Sniegoski, L. , Welch, M. , Phinney, K. and Zhang, N. (2010), Certification of Drugs of Abuse in a Human Serum Standard Reference Material: SRM 1959, Analytical and Bioanalytical Chemistry (Accessed July 13, 2024)


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Created March 24, 2010, Updated February 19, 2017