Cellular Response to Phase-Separated Blends of Tyrosine-Derived Polycarbonate

Published

March 1, 2006

Author(s)

L A. Bailey, Matthew Becker, J S. Stephens, Nathan D. Gallant, Christine M. Mahoney, N Washburn, Aarti Rege, J Kohn, Eric J. Amis

Abstract

Tyrosine-derived polycarbonates are extremely promising degradable materials for use in orthpedic applications and two-dimensional thin films consisting of homopolymer and discrete compositional blends of the ethyl (DTE) and octyl (DTO) ester polycarbonate derivatives were prepared as substrates to assist in the development of in vitro biocompatability metrologies. The homopolymer films were amorphous and smooth while the blends phase separated upon annealing with the domain size dependent on the composition. The surfaces were characterized by numerous methods in an effort to elucidate the nature of different cell responses that were measured in vitro. Genetic expression profiles of MC3T3-E1 osteoblasts and Raw 264.7 murine macrophages were measured at several time point providing small glimpses of the time dependent nature of the cell responses with regard to interleukin 1b and fibronectin production.The results of the discrete blend analyses have laid the foundation for the 1-D and 2-D gradients of DTE and DTO to examine further the optimal composition and processing conditions that maximize extracellular matrix production and minimize interleukin-1b response.

Citation

Journal of Biomedical Materials Research

Volume

76A

Pub Type

Journals

Keywords

biomaterials, fibronectin, phase separation, real-time polymerase chain reaction, tyosine-derived polycarbonate

Citation

Bailey, L. , Becker, M. , Stephens, J. , Gallant, N. , Mahoney, C. , Washburn, N. , Rege, A. , Kohn, J. and Amis, E. (2006), Cellular Response to Phase-Separated Blends of Tyrosine-Derived Polycarbonate, Journal of Biomedical Materials Research (Accessed October 14, 2025)