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Cell Seeding into Calcium Phosphate Cement



Carl Simon Jr., William F. Guthrie, Francis W. Wang


We have sought to improve calcium phosphate cement by developing a method for seeding bone cells into the cement. Calcium phosphate cement (CPC) is a dry white powder of calcium phosphate salts that when mixed with water will react to form microcrystalline hydroxyapatite (HA) in about 30 min. The cement is used clinically as a bone graft for dental and craniofacial applications and its primary advantage is that it forms a workable paste that can be sculpted to fit the contours of a wound before it hardens into HA. Over time, the hardened CPC is converted to new bone (osseoconversion) by bone cells. We have sought to shorten the conversion time of the CPC by developing a method to seed live bone cells into the cement. The presence of bone cells in the cement would allow osseoconversion to begin throughout the implant as soon as the CPC is implanted. Initial experiments showed that the setting reaction of the CPC paste (but not hardened CPC) kills cells. Thus, we have tested the ability of alginate to protect cells from the CPC paste during its 30 min setting reaction.
Journal of Biomedical Materials Research Part A
No. 4


alginate, bone graft, calcium phosphate cement


Simon Jr., C. , Guthrie, W. and Wang, F. (2004), Cell Seeding into Calcium Phosphate Cement, Journal of Biomedical Materials Research Part A, [online], (Accessed April 15, 2024)
Created February 29, 2004, Updated October 12, 2021