Capillary Electrophoretic Determination of Oxalate in Amniotic Fluid

Published

April 20, 1999

Author(s)

B C. Nelson, G F. Rockwell, T Campfield, P O'Grady, R M. Hernandez, Stephen Wise

Abstract

A capillary electrophoretic (CE) assay for oxalate has been applied to the quantitative determination of free oxalate in amniotic fluid. Indirect absorbance detection of oxalate is accomplished with a chromate-based background electrolyte modified with ethylenediaminetetraacetic acid (EDTA). Detection interference due to the presence of high levels (approximately equal to} 4 mg/ml) of inorganic chloride is eliminated through a direct sample clean-up procedure based on cation (Ag⁺-form) resins. Separation interference from amniotic fluid proteins is prevented through the use of a simple aqueous-based dilution procedure. This method for the determination of oxalate in amniotic fluid provides precision of approximately equal to} 5 % relative standard deviation (RSD). Within-day precisions for the oxalate peak response and migration time are better than 3% RSD and 1% RSD, respectively. Between-day precisions for the oxalate response and migration time are better than 6% RSD and 3% RSD, respectively. The analytical recovery of oxylate (1000 ng/ml) spiked into amniotic fluid was better than 96%. The limit of detection (LOD) for the method is approximately equal to} 100 ng/ml oxalate. This method also shows promising results for the determination of oxalate in human blood plasma samples.

Citation

Analytica Chimica Acta

Volume

410

Issue

1-2

Pub Type

Journals

Keywords

amniotic fluid, biological fluids, blood plasma, capillary electrophoresis, oxalate, total parenteral nutrition solutions

Citation

Nelson, B. , Rockwell, G. , Campfield, T. , O'Grady, P. , Hernandez, R. and Wise, S. (1999), Capillary Electrophoretic Determination of Oxalate in Amniotic Fluid, Analytica Chimica Acta (Accessed May 18, 2024)