Blood vessel-on-a-chip examines biomechanics of microvasculature
Paul Salipante, Steven D. Hudson, Styliani Alimperti
We use a three-dimensional (3D) microvascular platform to measure the elasticity and membrane permeability of the endothelial cell layer. The microfluidic platform is connected with a pneumatic pressure controller to apply hydrostatic pressure. The deformation is measured by tracking the mean vessel diameter under varying pressures up to 300 Pa. We obtain a value for the Young's modulus of the cell layer in low strain where a linear elastic response is observed and use a hyperelastic model that describes the strain hardening observed at larger strains (pressure). A fluorescent dye is used to track the flow through the cell layer to determine the membrane flow resistance as a function of applied pressure. Finally, we track the 3D positions of cell nuclei while the vessel is pressurized to observe local deformation and correlate inter-cell deformation with the local structure of the cell layer. This approach is able to probe the mechanical properties of blood vessels in vitro and provides a methodology for investigating microvascular related diseases.
, Hudson, S.
and Alimperti, S.
Blood vessel-on-a-chip examines biomechanics of microvasculature, Soft Matter, [online], https://doi.org/10.1039/D1SM01312B , https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933119
(Accessed February 26, 2024)