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Biodegradable biliverdin nanoparticles for efficient photoacoustic imaging



Parinaz Fathi, Knox J. Hailay, Dinabandhu Sar, Indu Tripathi, Ostadhossein Fatemeh, Misra K. Santosh, Mandy Esch, Jefferson Chan, Dipanjan Pan


Here we report the synthesis of nanoparticles composed of Biliverdin cross-linked with a bifunctional amine functionalized linker. The nanoparticles display high absorbance at 365 nm and 680 nm, fluorescence when excited at 365 nm, and photoacoustic properties when excited at 680 nm. Nanoparticles were shown to form as early as ten minutes into the synthesis, and their properties varied based on the synthesis solvent. Nanoparticles synthesized in water and containing a mass fraction of 0.9 % NaCl solution were found to exhibit higher absorbance and lower fluorescence compared to the nanoparticles synthesized in MES. Additionally, nanoparticles synthesized in MES exhibited a red-shifted fluorescence over time compared to the other nanoparticles, while nanoparticles synthesized in NaCl exhibited the strongest photoacoustic signal. The nanoparticles were found to be biodegradable, degrading in the presence of Biliverdin Reductase. The potential of these nanoparticles for use as photoacoustic agents in lymph node imaging was demonstrated in nude mice.
Angewandte Chemie-International Edition


Fathi, P. , Hailay, K. , Sar, D. , Tripathi, I. , Fatemeh, O. , Santosh, M. , Esch, M. , Chan, J. and Pan, D. (2019), Biodegradable biliverdin nanoparticles for efficient photoacoustic imaging, Angewandte Chemie-International Edition, [online],, (Accessed May 18, 2024)


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Created June 26, 2019, Updated October 12, 2021