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Application of Ion-Impact Energy Measurement to Electrospray Ionization Mass Spectrometry of Proteins and Protein Mixtures

Published

Author(s)

B. Neuhauser, Gene C. Hilton, John M. Martinis

Abstract

We have used normal metal-insulator-superconductor (NIS) microcalorimeter to measure the impact energy of protein ions produced by electrospray ionization (ESI) in a magnetic-sector mass spectrometer (MS). We have used these measurements to resolve spectral ambiguities and to analyze protein mixtures. Energy measurement may be useful for the direct MS analysis of complex biopolymer mixtures that normally would confound ESI-MS deconvolution algorithms.
Citation
Journal of the American Society for Mass Spectrometry
Volume
12

Citation

Neuhauser, B. , Hilton, G. and Martinis, J. (2001), Application of Ion-Impact Energy Measurement to Electrospray Ionization Mass Spectrometry of Proteins and Protein Mixtures, Journal of the American Society for Mass Spectrometry (Accessed May 29, 2024)

Issues

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Created December 31, 2000, Updated October 12, 2021