Application of Falling-Needle Rheometry to Highly Concentrated DNA Solutions.

Published

March 18, 2014

Author(s)

Jessica L. Burger, Hideki Yamamoto, Takamasa Suzuki, Arno D. Laesecke

Abstract

High concentration DNA solutions are common both in vitro and in vivo, and understanding the rheological properties is a critical area of bioscience. Our previous measurements on high concentration DNA solutions (2-6 mg/ml) interestingly provided evidence for a viscosity maximum with temperature in both the Ubbelohde capillary viscometer and rotating concentric cylinder viscometer which could be represented by a two-liquids model for solutions of double-and single-stranded DNA. In the work presented here we have expanded on our previous research and utilized a falling-needle rheometer in order to gain more insight into the nature of the previously observed viscosity maxima. We have shown that this viscosity increase is highly dependent on shear stress.

Citation

Biorheology

Volume

51

Issue

1

Pub Type

Journals

Download Paper

DOI Link

Keywords

Calf thymus DNA, falling-needle rheometer, yield stress, melting temperature, and viscosity.

Citation

Burger, J. , Yamamoto, H. , Suzuki, T. and Laesecke, A. (2014), Application of Falling-Needle Rheometry to Highly Concentrated DNA Solutions., Biorheology, [online], https://doi.org/10.3233/BIR-140650 (Accessed December 6, 2024)