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Accurate optical analysis of single molecule entrapment in nanoscale vesicles

Published

Author(s)

Joseph E. Reiner, Andreas Jahn, Samuel M. Stavis, Michael J. Culbertson, Wyatt N. Vreeland, Daniel L. Burden, Jon C. Geist, Michael Gaitan

Abstract

We present a non-destructive method to characterize low analyte concentrations in nanometer scale lipid vesicle formulations. Our method is based on the application of fluorescence fluctuation analysis (FFA) and multi-angle laser light scattering (MALLS) in conjunction with asymmetric field flow fractionation (AFFF) to measure the entrapment efficiency (defined here as the ratio of the concentration of encapsulated dye to the initial concentration of dye in the surrounding buffer) of an ensemble of nanoscale (D
Citation
Analytical Chemistry

Keywords

optical microscopy, liposomes, nanoscale characterization, fluorescence correlation spectroscopy
Created January 1, 2010, Updated February 19, 2017