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Accurate optical analysis of single molecule entrapment in nanoscale vesicles



Joseph E. Reiner, Andreas Jahn, Samuel M. Stavis, Michael J. Culbertson, Wyatt N. Vreeland, Daniel L. Burden, Jon C. Geist, Michael Gaitan


We present a non-destructive method to characterize low analyte concentrations in nanometer scale lipid vesicle formulations. Our method is based on the application of fluorescence fluctuation analysis (FFA) and multi-angle laser light scattering (MALLS) in conjunction with asymmetric field flow fractionation (AFFF) to measure the entrapment efficiency (defined here as the ratio of the concentration of encapsulated dye to the initial concentration of dye in the surrounding buffer) of an ensemble of nanoscale (D
Analytical Chemistry


optical microscopy, liposomes, nanoscale characterization, fluorescence correlation spectroscopy


Reiner, J. , Jahn, A. , Stavis, S. , J., M. , Vreeland, W. , Burden, D. , Geist, J. and Gaitan, M. (2010), Accurate optical analysis of single molecule entrapment in nanoscale vesicles, Analytical Chemistry (Accessed April 21, 2024)
Created January 1, 2010, Updated February 19, 2017