Accurate optical analysis of single molecule entrapment in nanoscale vesicles
Joseph E. Reiner, Andreas Jahn, Samuel M. Stavis, Michael J. Culbertson, Wyatt N. Vreeland, Daniel L. Burden, Jon C. Geist, Michael Gaitan
We present a non-destructive method to characterize low analyte concentrations in nanometer scale lipid vesicle formulations. Our method is based on the application of fluorescence fluctuation analysis (FFA) and multi-angle laser light scattering (MALLS) in conjunction with asymmetric field flow fractionation (AFFF) to measure the entrapment efficiency (defined here as the ratio of the concentration of encapsulated dye to the initial concentration of dye in the surrounding buffer) of an ensemble of nanoscale (D
, Jahn, A.
, Stavis, S.
, J., M.
, Vreeland, W.
, Burden, D.
, Geist, J.
and Gaitan, M.
Accurate optical analysis of single molecule entrapment in nanoscale vesicles, Analytical Chemistry
(Accessed May 24, 2022)