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3D Cellular Morphometrics With Multi-Scale Topographical Constraint



A Sehgal, Alamgir Karim, Eric J. Amis


The three dimensional cytoplasmic spread of osteoblast like MC3T3-E1 cells with substrate topography was investigated by atomic force microscopy (AFM). The analysis of AFM images as applied to an ensemble of cells from programmed multiple scans under identical test conditions, provide distributions of measurable cell shape functionals. Moments analysis procedures, analogous to treatments of polymer chain conformation in solution, were extended to quantify cell populations in terms of metrics of the projected cell area and distribution of cytoplasmic mass about the centroid. These mathematical analyses led to the discovery that the radius of gyration (Rg) of the cells was sharply defined for cell populations and changed sensitively with substrate chemistry and topography. We suggest that the use of Rg as a quantitative descriptor of cell shape may be generally applicable as important metric of cell response to cues from the biomaterial interface.
Cytometry Part A


atomic force microscopy, cell morphology, cell shape functionals, radius of gyration osteoblast, three dimensional moments analysis


Sehgal, A. , Karim, A. and Amis, E. (2008), 3D Cellular Morphometrics With Multi-Scale Topographical Constraint, Cytometry Part A (Accessed June 15, 2024)


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Created October 16, 2008