Lisa Stabryla (Assoc)

Lisa Stabryla joined the Complex Microbial Systems Group in 2021 as an NRC Postdoctoral Research Associate working with Jason Kralj. Her doctoral research focused on informing the design of engineered nanomaterials as next-generation antimicrobial agents, where she applied traditional microbiological methods, advanced molecular characterization techniques, and classic laboratory evolution to uncover underlying mechanisms of bacterial inactivation and resistance to silver nanoparticles as well as the evolution of these mechanisms with changes in nanomaterial physicochemical properties (i.e., size, shape, surface chemistry). (see “Unraveling a mechanism of bacterial resistance to silver nanoparticles” and “Silver nanoparticles, a sustainable antimicrobial?” for more information).

At NIST, Lisa’s research focuses on using molecular and culture-based methods to evaluate evolution of (and mechanisms for) antibiotic resistance in pathogenic environmental and clinical microbiota with the goal of understanding the influence of vertical gene transfer in the development of antibiotic resistance in the environment and informing new wastewater monitoring targets as early indicators of resistance.

AWARDS AND FUNDING

National Research Council Postdoctoral Fellowship, 2021 – Present
National Defense Science and Engineering Graduate Fellowship, 2017 – 2021

PUBLICATIONS

1. Stabryla, L.M., Clark, R., Gilbertson, L.M. (2022) Assessment of Using Design Thinking to Foster Creativity in an Undergraduate Sustainable Engineering Course. Advances in Engineering Education. Accepted.
2. Stabryla, L.M., Johnston, K.A., Diemler, N.A., Cooper, V.S., Millstone, J.E., Haig, S-J., Gilbertson, L.M. (2021). Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions. Nature Nanotechnology, 16, 996-1003.  https://doi.org/10.1038/s41565-021-00929-w. 
3. Albalghiti, E., Stabryla, L.M., Gilbertson, L.M., Zimmerman, J.B. (2021) Towards resolution of antibacterial mechanisms in metal and metal oxide nanomaterials: a meta-analysis of the influence of study design on mechanistic conclusions. Environmental Science: Nano, 8, 37-66. https://doi.org/10.1039/D0EN00949K.
4. Clark, R., Stabryla, L.M., Gilbertson, L.M. (2020) Sustainability coursework: student perspectives and reflections on design thinking. International Journal of Sustainability in Higher Education, 21(3): 593-611. https://doi.org/10.1108/IJSHE-09-2019-0275.
5. Johnston, K.A., Stabryla, L.M., Gilbertson, L.M., Millstone, J.E. (2019) Emerging investigator series: connecting concepts of coinage metal stability across length scales. Environmental Science: Nano, 6, 2674-2696. https://doi.org/10.1039/C9EN00407F.
6. Stabryla, L.M., Johnston, K.A., Millstone, J.E., Gilbertson, L.M. (2018) Emerging investigator series: it’s not all about the ion: support for particle-specific contributions to silver nanoparticle antimicrobial activity. Environmental Science: Nano, 5(9), 2047-2068. https://doi.org/10.1039/c8en00429c.
7. Johnston, K.A., Stabryla, L.M., Smith, A.M., Gan, X.Y., Gilbertson, L.M., Millstone, J.E. (2018) Impacts of broth chemistry on silver ion release, surface chemistry composition, and bacterial cytotoxicity of silver nanoparticles. Environmental Science: Nano, 5, 304-312. https://doi.org/10.1039/C7EN00974G.
8. Clark, R., Stabryla, L.M., Gilbertson, L.M. (2018) Use of active learning and the design thinking process to drive creative sustainable engineering design solutions. American Society for Engineering Education (ASEE) 125^th Annual Conference & Exposition.  
9. Geng, H., Feng, J., Stabryla, L.M., Cho, S.K. (2017) Dielectrowetting manipulation for digital microfluidics: creating, transporting, splitting, and merging of droplets. Lab on a Chip, 17(6), 1060-1068. https://doi.org/10.1039/C7LC00006E.
10. Cowan, D.M., Maskrey, J.R., Fung, E.M., Woods, T.A., Stabryla, L.M., Scott, P.K., Finley, B.L. (2016) Best-practices approach to determination of blood alcohol concentration (BAC) at specific time points: combination of ante-mortem alcohol pharmacokinetic modeling and post-mortem alcohol generation and transport considerations. Regulatory Toxicology and Pharmacology, 78, 24-36. https://doi.org/10.1016/j.yrtph.2016.03.020.
11. Chen, Y., Stabryla, L.M., Wei, N. (2016) Improved acetic acid resistance in Saccharomyces Cerevisiae by overexpression of the WHI2 gene identified through inverse metabolic engineering. Applied and Environmental Microbiology, 82, 2156-2166. https://doi.org/10.1128/AEM.03718-15.

PROFESSIONAL ORGANIZATIONS

American Society for Microbiology, 2019 – Present                                                                 
Association of Environmental Engineering and Science Professors, 2019 – Present