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Enhancing Optical/Electron Phase Microscopy for Biological Applications

Optical phase contrast microscopy and cryo-electron microscopy are widely used in the study of cells and proteins, respectively. In both techniques, a specimen imparts a phase shift on the probing particles (photons or electrons), which can be measured using various interferometric techniques.
In this talk I will briefly discuss the physical basics and limits of phase microscopy, and will show ways how to improve on current techniques using wave-front shaping, cavity or quantum enhanced measurements.
I will demonstrate how wave-front shaping can enable phase contrast imaging with optimized sensitivity all across the field of view, and how multi-passing the probe particles through a sample multiple times can be used for high sensitivity / low damage imaging [1]. The latter could potentially allow for cryo-electron microscopy with unprecedented resolution [2].
 
                       [1]                      T.Juffmann, B.B.Klopfer, T.L.Frankort, P.Haslinger & M.A.Kasevich, Nature Communications 7, 12858 (2016)
                       [2]                      T.Juffmann, S.A.Koppell, B.B.Klopfer, C.Ophus, R.M.Glaeser & M.A.Kasevich, Scientific Reports 7, 1699 (2017)

Thomas Juffmann

Stanford University

Created March 29, 2018, Updated October 1, 2018