Molecular characterization

News and Updates

NIST Puts New Biopharmaceutical Quality Control Method to the Test

March 29, 2021

A new method for monitoring biopharmaceutical product quality was recently put to the test by The National Institute of Standards and Technology (NIST) and 28

NIST Publishes a Beginner’s Guide to DNA Origami

January 8, 2021

In a technique known as DNA origami, researchers fold long strands of DNA over and over again to construct a variety of tiny 3D structures, including miniature

Making the DNA Melt Curve More Accurate

August 18, 2020

DNA is not only the blueprint of life; it has become the backbone for making tiny structures that can be inserted into the human body to diagnose and treat

Projects and Programs

NCNR DAC and CCUS efforts

Overview Capture of carbon dioxide from the air (direct air capture; DAC) combined with energy efficiency and carbon capture, utilization and storage (CCUS)

Platform for Realizing Integrated Molecule Experiments (PRIME)

PRIME (Platform for Realizing Integrated Molecule Experiments) aims to establish primary, quantum-based realizations for both the radiometric watt and kelvin

Materials Extrusion Additive Manufacturing

Polymer Additive Manufacturing and Rheology

Additive manufacturing (AM) is a process for fabricating parts directly from 3-D digital models which has tremendous potential for producing high-value, complex

Novel Psychoactive Substance (NPS) Data Hub

NIST is collaborating with the DEA and the Federal Criminal Police Office of Germany – Bundeskriminalamt (BKA) to develop a web-based forensic drug chemistry

Publications

Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb and Their Use for Developing a Mass Spectral Library

June 22, 2021

Author(s)

Qian Dong, Xinjian Yan, Yuxue Liang, Sanford Markey, Sergey L. Sheetlin, Concepcion Remoroza, William E. Wallace, Stephen Stein

This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb. Analysis

Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the FcγR1a (CD64) Receptor

June 17, 2021

Author(s)

Kyle Anderson, Kerry Scott, Ioannis Karageorgos, Elyssia Gallagher, Venkata Tayi, Michael Butler, Jeffrey W. Hudgens

This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F

CID fragmentation of deprotonated N-acyl aromatic sulfonamides. Smiles-type and nitrogen-oxygen rearrangements

May 19, 2021

Author(s)

Yuxue Liang, Yamil Simon, Pedatsur Neta, Xiaoyu Yang, Stephen Stein

The NIST tandem mass spectral library (2020 version) includes over 800 aromatic sulfonamides. In negative mode, upon collisional activation most

Compensation of Strong Water Absorption in Infrared Spectroscopy Reveals the Secondary Structure of Proteins in Dilute Solutions

January 12, 2021

Author(s)

Bonghwan Chon, Shuyu Xu, Young J. Lee

Infrared (IR) absorption spectroscopy is a powerful tool that can quantify complex biomolecules and their structural conformations. However, conventional

Tools and Instruments

Fourier Transform Infrared Spectroscopy

Fourier Transform Infrared (FTIR) Spectroscopy is a spectroscopic technique in which the vibrational modes of the molecules comprising a material of interest