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Search Publications by: Mark Lowenthal (Fed)

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Displaying 1 - 25 of 32

Comparison of N-glycopeptide to released N-glycan abundances and the influence of glycopeptide mass and charge state on N-linked glycosylation of IgG antibodies

March 7, 2024
Author(s)
Concepcion Remoroza, Meghan Burke Harris, Tytus Mak, Sergey Sheetlin, Yuri Mirokhin, Zachary Goecker, Brian T. Cooper, Mark Lowenthal, Xiaoyu (Sara) Yang, Guanghui Wang, Dmitrii V. Tchekhovskoi, Stephen E. Stein
We report the comparison of mass-spectral-based abundances of tryptic glycopeptides to fluorescence abundances of released labeled glycans and the effects of mass and charge state and in-source fragmentation on glycopeptide abundances. The primary

Quantification of mRNA in Lipid Nanoparticles Using Mass Spectrometry

December 26, 2023
Author(s)
Mark Lowenthal, Abigail Antonishek, Karen W. Phinney
Lipid nanoparticle-encapsulated messenger RNA (LNP-mRNA) holds great promise as a novel modality for treating a broad range of diseases. The ability to quantify mRNA accurately in therapeutic products helps to ensure consistency and safety. Here we

Development of an Improved Standard Reference Material for Folate Vitamers in Human Serum

December 12, 2022
Author(s)
Johanna Camara, Jeanita Pritchett, Yasmine Daniels, Mary Bedner, Michael Nelson, Mark Lowenthal, Zia Fazili, Christine Pfeiffer, Karen W. Phinney, Katherine E. Sharpless, Lane C. Sander, Katrice Lippa, James H. Yen, Adam Kuszak, Stephen Wise
The US National Institute of Standards and Technology (NIST) developed a Standard Reference Material® (SRM®) 3949 Folate Vitamers in Frozen Human Serum to replace SRM 1955 Homocysteine and Folate in Human Serum. The presence of increased endogenous levels

Certification of Standard Reference Material(R) 3655 Glycans in Solution (Frozen)

April 12, 2022
Author(s)
Mark Lowenthal, Blaza Toman, Brian Lang, Karen W. Phinney
Standard Reference Material (SRM) 3655 is intended primarily for use as a calibration standard for the measurement of enzymatically released N-linked glycans. Applications of SRM 3655 include the benchmarking and comparability of analytical techniques, as

Development of an LC-MS/MS Reference Method for the Standardization of Analytical Methods to Measure Lipoprotein(a) in Plasma

April 1, 2021
Author(s)
Santica Marcovina, Noemie Clouet-Foraison, Marlys Koschinsky, Mark Lowenthal, Michael Boffa, Andrew Hoofnagle, Tomas Vaisar
Background: Use of Lipoprotein(a) levels for identification of individuals at high risk for cardiovascular disease is hampered by the high size polymorphism of apolipoprotein(a) which strongly impacts immunochemical methods resulting in discordant values

Absolute quantification of RNA or DNA using acid hydrolysis and mass spectrometry

October 22, 2019
Author(s)
Mark S. Lowenthal, Eva Quittman, Karen W. Phinney
Accurate, traceable quantification of ribonucleotide or deoxyribonucleotide oligomers is achievable using acid hydrolysis and isotope dilution mass spectrometry (ID-MS). In this work, formic acid hydrolysis is demonstrated to generate stoichiometric

The integration of emerging omics approaches to advance precision medicine: How can regulatory science help?

December 6, 2018
Author(s)
Mark S. Lowenthal, Christina M. Jones, Joan Adamo, Robert Bienvenu, Owen Fields, Soma Ghosh, Michael Liebman, Robert Schuck, Scott Steele
Building on the recent advances in next-generation sequencing, the integration of genomics, proteomics, metabolomics, and other approaches hold tremendous promise for precision medicine. The approval and adoption of these rapidly advancing technologies and

Harmonization of LC-MS/MS protein assays

September 24, 2018
Author(s)
Alan L. Rockwood, Mark Lowenthal, Cory Bystrom
Harmonization of diagnostic test results is fundamental to the effective use of laboratory testing in the diagnosis, treatment and monitoring of disease. Formal approaches to harmonization and standardization provide a rigorous and high quality Roadmap to

Identification of novel N-glycosylation sites at non-canonical protein consensus motifs

June 14, 2016
Author(s)
Mark S. Lowenthal, Kiersta S. Davis, Lisa E. Kilpatrick, Catherine A. Mouchahoir, Karen W. Phinney
N-glycosylation is well known to occur at asparagine residues in the canonical consensus sequence N-X-S/T, but has also been identified at a small number of N-X-C motifs including the Asn491 residue of human serotransferrin. Here we report additional novel

MEASUREMENT OF DNA REPAIR PROTEINS IN RELATION TO DISEASE BIOMARKERS AND DRUG DEVELOPMENT

November 7, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Prasad T. Reddy, M Miral Dizdar, Ann-Sofie Jemth, Olga Loseva, Thomas Helleday
Introduction: In aerobic organisms, intracellular metabolism and exogenous sources such as ionizing radiation and carcinogenic compounds generate reactive species including free radicals derived from either oxygen or nitrogen. Oxidative stress thereby

Extreme Expressions of DNA Repair Proteins APE1 and MTH1, in Human Breast Cancer as Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry

September 15, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Prasad T. Reddy, M Miral Dizdar, Ann-Sofie Jemth, Olga Loseva, Thomas Helleday
Accurate measurement of DNA repair proteins in cancer tissues is becoming more important due to the individual and origin based expression differences in cancer patients as well as the novel approach of using the repair enzyme inhibitors in cancer

Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements

July 26, 2015
Author(s)
Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday, Miral M. Dizdar
Oxidatively induced DNA damage is caused in living organisms by a variety of damaging agents, resulting in the formation of a multiplicity of lesions, which are mutagenic and cytotoxic. Unless repaired by DNA repair mechanisms before DNA replication, DNA

Addiction to MTH1 protein results in intense expression in human breast cancer tissue as measured by liquid chromatography-isotope-dilution tandem mass spectrometry

June 23, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Ann-Sofie Jemth, Olga Loseva, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Thomas Helleday, M Miral Dizdar
MTH1 protein sanitizes the nucleotide pool so that modified 2'-deoxynucleoside triphosphates (dNTPs) cannot be used in DNA replication. Cancer cells require MTH1 to avoid incorporation of modified dNTPs resulting in DNA damage mutations and cell death

Quantitative bottom-up proteomics depends on digestion conditions

December 2, 2013
Author(s)
Mark S. Lowenthal, Yuxue Liang, Karen W. Phinney, Stephen E. Stein
Accurate quantification is a fundamental requirement in the fields of proteomics and biomarker discovery, and for clinical diagnostic assays. To demonstrate the extent of quantitative variability in measurable peptide concentrations due to differences

Development of a Standard Reference Material for Metabolomics Research

November 4, 2013
Author(s)
Karen W. Phinney, Guillaume Ballihaut, Mary Bedner, Johanna Camara, Steven J. Christopher, William C. Davis, Nathan G. Dodder, Brian E. Lang, Stephen E. Long, Mark S. Lowenthal, Elizabeth A. McGaw, Karen E. Murphy, Bryant C. Nelson, Jocelyn L. Prendergast, Jessica L. Reiner, Catherine A. Rimmer, Lane C. Sander, Michele M. Schantz, Katherine E. Sharpless, Lorna T. Sniegoski, Susan S. Tai, Jeanice M. Brown Thomas, Thomas W. Vetter, Michael J. Welch, Stephen A. Wise, Laura J. Wood, William F. Guthrie, Robert C. Hagwood, Stefan D. Leigh, James H. Yen, Nien F. Zhang, Madhu Chaurhary-Webb, Huiping Chen, Bridgette Haynes, Donna J. LaVoie, Leslie F. McCoy, Shahzad S. Momin, Neelima Paladugula, Elizabeth C. Pendergrast, Christine M. Pfeiffer, Carissa D. Powers, Zia Fazili-Qari, Daniel Rabinowitz, Michael E. Rybak, Rosemary L. Schleicher, Mary Xu, Mindy Zhang, Arthur L. Castle, Brandi S. Benford, Gauthier Eppe

Metabolite Profiling of a NIST Standard Reference Material for Human Plasma (SRM 1950) – GC/MS, LC/MS, NMR and Clinical Laboratory Analyses, Libraries and Web-based resources

October 22, 2013
Author(s)
Yamil Simon, Mark S. Lowenthal, Lisa E. Kilpatrick, Maureen L. Sampson, Kelly H. Telu, Paul A. Rudnick, William G. Mallard, Daniel W. Bearden, Tracey B. Schock, Dmitrii V. Tchekhovskoi, Niksa Blonder, Xinjian Yan, Yuxue Liang, Yufang Zheng, William E. Wallace, Pedatsur Neta, Karen W. Phinney, Alan T. Remaley, Stephen E. Stein
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest in global profiling, i.e., semi-quantitative monitoring of all chemical constituents of biological fluids. In this work, we have