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You searched on: Topic Area: Life Sciences Research

Displaying records 171 to 180 of 273 records.
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171. RNA Polymerase-cNMP-Ligated cAMP Receptor Protein (CRP) Mutant Interactions in the Enhancement of Transcription by CRP Mutants
Topic: Life Sciences Research
Published: 10/27/2000
Authors: S. Wang, Y. Shi, I Gorshkova, Frederick P. Schwarz
Abstract: The enhancement of the transcription of three synthetic promoters by cNMP-ligated cAMP receptor protein (CRP)/mutant complexes was determined from the transcription yields of a short AAUU transcript in an abortive initiation in vitro transcription as ...

172. Stressing-Out DNA? The Contribution of Serine-Phosphodiester Interactions in Catalysis by Uracil DNA Glycosylase
Topic: Life Sciences Research
Published: 10/17/2000
Authors: R M. Werner, Y. L. Jiang, R. G. Gordley, J. Jagadeesh, Jane E Ladner, G Xiao, M Tordova, G L. Gilliland, J T. Stivers
Abstract: The DNA repair enzyme uracil DNA glycosylase (UDG) pinches the phosphodiester backbone of damaged DNA using the hydroxyl side chains of a conserved trio of serine residues, resulting in flipping of the deoxyuridine from the DNA helix into the enzyme ...

173. Prokaryotic Expression, Purification, and Reconstitution of Biological Activities (Antiprotease, Antitumor and Heparin-Binding) for Tissue Factor Pathway Inhibitor- 2
Topic: Life Sciences Research
Published: 10/5/2000
Authors: C N Rao, Prasad T Reddy, D J. Reeder, Yonglin Liu, S M Stack, W Kisiel, D T Woodley
Abstract: We report the expression of tissue factor pathway inhibitor-2 (TFPI-2) (also known as PP-5, placental protein-5; MSPI, matrix-associated serine protease inhibitor) in E. coli as a 25 kDa non-glycosylated protein with a glycine substituted for asparti ...

174. The Electronic Spectrum of the Prephenate Dianion. An Experimental and Theoretical (MD/QM) Comparison
Topic: Life Sciences Research
Published: 8/2/2000
Authors: A. E. Roitberg, S. E. Worthington, Marcia J Holden, M P. Mayhew, Morris Krauss
Abstract: Prephenate is the product of a Claisen rearrangement of chorismate. The enzyme chorismate mutase (CM from B. subtilis) accelerates the reaction by a factor of 10^u6^. The standard method for quantifying prephenate measures the electronic absorption ...

175. Cytochrome c at Model Membrane Surfaces: Exploration via Second Harmonic Generation - Circular Dichroism and Surface-Enhanced Resonance Raman Spectroscopy
Topic: Life Sciences Research
Published: 7/11/2000
Authors: T Petralli-Mallow, Anne L Plant, M Lewis, J Hicks
Abstract: The novel nonlinear optical method second harmonic generation-circular dichroism (SHG-CD) has been used to follow the adsorption and redox properties of a peripheral membrane protein horse heart cytochrome c, adsorbed at several model membrane surfac ...

176. Improving the Cytochrome P450 Enzyme System for Electrode-Driven Biocatalysis of Styrene Epoxidation
Topic: Life Sciences Research
Published: 7/1/2000
Authors: M P. Mayhew, Vytautas Reipa, Marcia J Holden, V L. Vilker
Abstract: Cytochrome P450 enzymes catalyze a vast array of oxidative and reductive biotransformations that are potentially useful for industrial and pharmaceutical syntheses. Factors such as cofactor utilization and slow reaction rates for non-natural substra ...

177. Intersubunit Association Induces Unique Allosteric Dependence of the T127L CRP Mutant on pH
Topic: Life Sciences Research
Published: 6/20/2000
Authors: Y. Shi, S. Wang, Frederick P. Schwarz
Abstract: The allosteric activation of the T127->L mutant of 3'-5' cyclic adenosine monophosphate (cAMP) receptor protein (CRP) by cAMP changes from an exothermic, independent two-site bindingmechanism at pH 7.0 to an endothermic, interacting two-site binding ...

178. Response to "Comment on 'Reinterpretation of the Spectra of Hydrated Co++: An Ab Initio Study'"
Topic: Life Sciences Research
Published: 6/15/2000
Authors: H S. Gilson, Morris Krauss
Abstract: This is a response to comments on a previous paper. Reinterpretation of the Spectra of Hydrated Co++: an ab Initio Study.

179. Effective Fragment Potentials and the Enzyme Active Site
Topic: Life Sciences Research
Published: 5/1/2000
Authors: S E Worthington, Morris Krauss
Abstract: Optimization of the binding conformation of a substrate in an enzyme active site using ab initio quantum chemistry methods are intractable since the active site comprises several hundred atoms. However, the active site can be decomposed into an activ ...

180. Spin-Orbit Configuration Interaction Calculation of the Potential Energy Curves of Iodine Oxide
Topic: Life Sciences Research
Published: 4/6/2000
Authors: S Roszak, Morris Krauss, A B Alekseyev, H P Liebermann, R J Buenker
Abstract: An ab initio cofiguration interaction (CI) study including spin-orbit coupling is carried out for the ground and excited states of the IO radical by employing relativistic effective core potentials. The computed spectroscopic constants are in good a ...

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