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NIST Standard Reference Database 1C

NIST Peptide Tandem Mass Spectral Libraries with Search Programs (Data Version: 2012, MS Search Version 2.0g, MSPepSearch Version 0.9)

(Libraries are available free of charge at http://peptide.nist.gov.  Note:  Not all libraries are updated each year.)


 

Contents of the Libraries

Library

Date

Instrument

Species

Spectra

Human

05-30-2012

it

H. sapiens

324,352

Human

04-20-2012

qtof

H. sapiens

16,010

Mouse

04-21-2012

it

M. musculus

143,418

Drosophila

04-11-2012

it

D. melanogaster

78,599

C. elegans

05-24-2011

it

C. elegans

67,470

Yeast

04-06-2012

it

S. cerevesiae

92,609

Yeast

04-17-2012

qtof

S. cerevesiae

14,647

E. coli

04-20-2012

it

E. coli

50,369

Rat

04-16-2012

it

R. norvegicus

55,668

Chicken

05-24-2011

it

chicken

3,125

SigmaUPS1

05-24-2011

it

standard

3,029

BSA

04-01-2011

it

Bovine

725



Compilation
· Data for building the libraries come from typical proteomic measurements of tryptic peptides
· Data are collected from internal measurements, public repositories and directly from producers
· UniRef100 protein sequences are used when available to make identifications
· Primary identifications of MS/MS spectra are made by multiple sequence search engines
· Consensus spectra are generated from a minimum of 3 and a maximum of 100 replicates
· Consensus spectra with high levels of impurities have been removed through an automated process
· The libraries are produced in ASCII .msp format, NIST binary folders and SpectraST formats

Library Search Sofware

· The full-featured software MS Search 2.0 is fully compatible with peptide libraries
· A batch search program, MSPepSearch, is also available

 nist1c

 

System Requirements: PC with Microsoft® Windows NT® 4.0, Windows® 2000, Windows® XP, Windows Vista®,or Windows® 7; hard disk with 1GB of available space required.

For more information please contact:

Standard Reference Data Program
National Institute of Standards and Technology
100 Bureau Dr., Stop 2300
 Gaithersburg, MD 20899-2300
(301) 975-2008 (VOICE)(301) 926-0416 (FAX) Contact Us (E-MAIL)

 

The Technical contacts for the database:

Stephen E. Stein
Mass Spectrometry Data Center
National Institute of Standards and Technology
Gaithersburg, MD 20899-8320
(301) 975-2505 sstein@nist.gov

Paul Rudnick
Chemical and Biochemical Reference Data Division 
National Institute of Standards and Technology
Gaithersburg, MD 20899
phone: (301) 975-5828 email: paul.rudnick@nist.gov


Keywords:
peptide mass spectral library, NIST peptide library, proteomics, peptide fragmentation, library search, library search.