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Topic Area: Life Sciences Research

Displaying records 11 to 20 of 271 records.
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11. Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics
Topic: Life Sciences Research
Published: 10/11/2013
Authors: Stephen E Stein, Paul A Rudnick, Scott J Walmsley, Qian Dong, Alexey I Nesvizhskii, Yuxue Liang
Abstract: Trypsin is an endoprotease commonly used for sample preparation in proteomics experiments. Importantly, protein digestion is dependent on multiple factors, including the trypsin origin and digestion conditions. In-depth characterization of tryp ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914042

12. Some Journal Metrics for Forensic Science International: Genetics
Topic: Life Sciences Research
Published: 10/2/2013
Author: John M Butler
Abstract: Forensic Science International: Genetics, which is the official journal of the International Society for Forensic Genetics, has grown in readership and impact factor since its introduction in 2007. This article reviews some metrics of the 32 issu ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914636

13. Total free energy governs cell morphology ‹ A possible solution to obtain intrinsic cell modulus
Topic: Life Sciences Research
Published: 9/26/2013
Authors: Martin Y Chiang, Yanzi Yangben , Nancy J Lin, Li Yang
Abstract: Cells adjust their morphology in response to physical and chemical interactions with their environment. In this report, we deem that the fundamental nature of free energies associated with cell/substrate interactions regulates adherent cell morpholog ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909671

14. Measurement Uncertainty in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7954
Topic: Life Sciences Research
Published: 8/13/2013
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Abstract: Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914171

15. Capturing Rare Cells From Blood Using a Packed Bed of Custom-Synthesized Chitosan Microparticles
Topic: Life Sciences Research
Published: 7/1/2013
Authors: Chandamany Arya, Jason G Kralj, Kunqiang Jiang, Matthew S Munson, Thomas P Forbes, Don L. DeVoe, Srinivasa R Raghavan, Samuel P Forry
Abstract: Uniform multifunctional chitosan microparticles were produced in large numbers using a simple and inexpensive microtubing arrangement. The particles were functionalized through both physical encapsulation of carbon black, to attenuate autofluores ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912723

16. Evaluation of Vitamin D Standardization Program protocols for standardizing serum 25-hydroxyvitamin D data: a case study of the program's potential for national nutrition and health surveys
Topic: Life Sciences Research
Published: 6/3/2013
Authors: Karen W Phinney, Kevin Cashman, Mairead Kiely, Michael Kinsella, Ramon Durazo-Arvizu, Lu Tian, Yue Zhang, Alice Lucey, Albert Flynn, Michael Gibney, Mary Frances Picciano, Christopher T. Sempos
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915282

17. Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins
Topic: Life Sciences Research
Published: 4/22/2013
Authors: Antony K. Chen, Frank W DelRio, Alexander W Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L Plant
Abstract: The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910923

18. Effect of Surface Chemistry on Stem Cell Response in 2-D vs. 3-D Cell Culture Niches
Topic: Life Sciences Research
Published: 4/19/2013
Authors: Sumona Sarkar, Carl George Simon Jr, Roberta I Lock, Joy P Dunkers
Abstract: To develop tissue engineered devices for regenerative medicine strategies, key scaffold properties for directing stem cell response must be identified. As focus shifts from 2-D to 3-D culture systems in an attempt to better recapitulate the na ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912888

19. Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1
Topic: Life Sciences Research
Published: 4/10/2013
Authors: Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant C Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Abstract: Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we p ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913265

20. The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes
Topic: Life Sciences Research
Published: 2/13/2013
Authors: Zvi Kelman, Kira S Makarova, Eugene V Koonin
Abstract: In eukaryotes, the CMG (Cdc45, MCM, GINS) complex functions as the replicative helicase and plays a key role in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the CDC4 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910056



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