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Topic Area: Bioscience
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Displaying records 1 to 10 of 459 records.
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1. Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-Stokes Raman scattering microscopy
Topic: Bioscience & Health
Published: 12/31/2014
Authors: Marcus T Cicerone, Young Jong Lee, Khaled A. Aamer, Prabhas V Moghe, Sebastian L Vega, Parth J Patel
Abstract: We use broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to characterize lineage commitment of individual human mesenchymal stem cells (hMSCs) cultured in adipogenic, osteogenic, and basal culture media. We treat hyperspectral i ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913019

2. Spectral broadening and shaping of nanosecond pulses: towards shaping of single photons from quantum emitters
Topic: Bioscience & Health
Published: 10/1/2014
Authors: Imad I. Agha, Serkan Ates, Luca Sapienza, Kartik A Srinivasan
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=916179

3. Physics-based Electro-thermal Saber Model and Parameter Extraction for High-Voltage SiC Buffer IGBTs
Topic: Bioscience & Health
Published: 9/15/2014
Authors: Tam Hoang Duong, Allen R Hefner Jr, Jose Miguel Ortiz, Sei-Hyung Ryu , Edward VanBrunt, Lin Cheng, Scott Allen, John W. Palmour
Abstract: The purpose of this paper is to present a physics-based electro-thermal Saber model and parameter extraction sequence for high-voltage SiC buffer layer n-channel insulated gate bipolar transistors (IGBTs). This model was developed by modifying and ex ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=916319

4. Rbm24a and Rbm24b are required for normal somitogenesis
Topic: Bioscience & Health
Published: 8/29/2014
Author: Samantha D Maragh
Abstract: We recently demonstrated that the gene encoding the RNA binding motif protein 24 (Rbm24)¬¬ is expressed during mouse cardiogenesis, and determined the developmental requirement for its zebrafish homologs Rbm24a and Rbm24b during cardiac developme ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915918

5. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Topic: Bioscience & Health
Published: 8/11/2014
Authors: Michael W Halter, Elianna Bier, Paul C DeRose, Gregory A Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Abstract: Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915188

6. Membrane protein resistance of oligo(ethylene oxide) self-assembled monolayers
Topic: Bioscience & Health
Published: 7/31/2014
Authors: David J Vanderah, Marlon L Walker, David Travis Gallagher, Ryan Vierling, Fay Crawshaw
Abstract: Spectroscopic ellipsometry was used to evaluate the resistance to protein adsorption (RPA) of self- assembled monolayers (SAMs) of HS(CH2)3O(CH2CH2O)6M and [HS(CH2)3CH]2O-(CH2CH2O)6M, where M = CH3 or H, on Au. The SAMs were exposed to fibrinogen ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913472

7. Viscosity Measurements of DNA Solutions with and without Condensing Agents
Topic: Bioscience & Health
Published: 7/7/2014
Authors: Arno R Laesecke, Jessica Lynne Burger
Abstract: Aqueous DNA solutions at three concentrations were studied as model sputum by viscosity measurements at ambient pressure. Initial measurements at 25 {degree}C, 37.78 {degree}C, and 50 {degree}C indicated a viscosity increase toward 50 {degree}C. Th ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914036

8. Tandem Mass Spectral Libraries of Peptides in Digests of Individual Proteins: Human Serum Albumin
Topic: Bioscience & Health
Published: 6/2/2014
Authors: Qian Dong, Xinjian Yan, Lisa Ervin Kilpatrick, Yuxue Liang, Yuri Alexander Mirokhin, Jeri S Roth, Stephen E Stein
Abstract: This work presents a method for creating a mass spectral library containing tandem spectra identifiable peptide ions in the tryptic digestion of a single protein. Human serum albumin (HSA) was selected for this purpose owing to its ubiquity and h ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915114

9. Standards in Biothermodynamics
Topic: Bioscience & Health
Published: 5/15/2014
Author: Robert Nathan Goldberg
Abstract: The field of biothermodynamics encompasses physical property measurements on biochemical and biological systems. This chapter reviews the status of standards documents that are pertinent to biothermodynamics as well as recommendations that have been ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908689

10. Measuring Organelle Shape in 3D in Stem Cells Cultured on Nanofiber Scaffolds
Topic: Bioscience & Health
Published: 4/19/2014
Authors: Carl George Simon Jr, Peter Bajcsy, Wojtek J Tutak, Jyotsnendu (Jyotsnendu) Giri
Abstract: Previous work has demonstrated that culture of osteoprogenitor cells on nanofiber scaffolds can potentiate osteogenic differentiation [1-4]. Culture of cells in nanofiber scaffolds causes changes to cell morphology, suggesting that morphological ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914995



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