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Displaying records 1 to 10 of 455 records.
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1. Membrane protein resistance of oligo(ethylene oxide) self-assembled monolayers
Topic: Bioscience & Health
Published: 7/31/2017
Authors: David J Vanderah, Marlon L Walker, David Travis Gallagher, Ryan Vierling, Fay Crawshaw
Abstract: Spectroscopic ellipsometry was used to evaluate the resistance to protein adsorption (RPA) of self- assembled monolayers (SAMs) of HS(CH2)3O(CH2CH2O)6M and [HS(CH2)3CH]2O-(CH2CH2O)6M, where M = CH3 or H, on Au. The SAMs were exposed to fibrinogen ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913472

2. Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-Stokes Raman scattering microscopy
Topic: Bioscience & Health
Published: 12/31/2014
Authors: Marcus T Cicerone, Young Jong Lee, Khaled A. Aamer, Prabhas V Moghe, Sebastian L Vega, Parth J Patel
Abstract: We use broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to characterize lineage commitment of individual human mesenchymal stem cells (hMSCs) cultured in adipogenic, osteogenic, and basal culture media. We treat hyperspectral i ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913019

3. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Topic: Bioscience & Health
Published: 8/11/2014
Authors: Michael W Halter, Elianna Bier, Paul C DeRose, Gregory A Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Abstract: Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915188

4. Viscosity Measurements of DNA Solutions with and without Condensing Agents
Topic: Bioscience & Health
Published: 7/7/2014
Authors: Arno R Laesecke, Jessica Lynne Burger
Abstract: Aqueous DNA solutions at three concentrations were studied as model sputum by viscosity measurements at ambient pressure. Initial measurements at 25 {degree}C, 37.78 {degree}C, and 50 {degree}C indicated a viscosity increase toward 50 {degree}C. Th ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914036

5. Tandem Mass Spectral Libraries of Peptides in Digests of Individual Proteins: Human Serum Albumin
Topic: Bioscience & Health
Published: 6/2/2014
Authors: Qian Dong, Xinjian Yan, Lisa Ervin Kilpatrick, Yuxue Liang, Yuri Alexander Mirokhin, Jeri S Roth, Stephen E Stein
Abstract: This work presents a method for creating a mass spectral library containing tandem spectra identifiable peptide ions in the tryptic digestion of a single protein. Human serum albumin (HSA) was selected for this purpose owing to its ubiquity and h ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915114

6. Standards in Biothermodynamics
Topic: Bioscience & Health
Published: 5/15/2014
Author: Robert Nathan Goldberg
Abstract: The field of biothermodynamics encompasses physical property measurements on biochemical and biological systems. This chapter reviews the status of standards documents that are pertinent to biothermodynamics as well as recommendations that have been ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908689

7. Measuring Organelle Shape in 3D in Stem Cells Cultured on Nanofiber Scaffolds
Topic: Bioscience & Health
Published: 4/19/2014
Authors: Carl George Simon Jr, Peter Bajcsy, Wojtek J Tutak, Jyotsnendu (Jyotsnendu) Giri
Abstract: Previous work has demonstrated that culture of osteoprogenitor cells on nanofiber scaffolds can potentiate osteogenic differentiation [1-4]. Culture of cells in nanofiber scaffolds causes changes to cell morphology, suggesting that morphological ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914995

8. Roles of Nanofiber Structure and Chemistry in Directing Stem Cell Morphology, Differentiation and Gene Expression
Topic: Bioscience & Health
Published: 4/19/2014
Authors: Sumona Sarkar, Bryan A. Baker, Desu Chen, Wolfgang Losert, Carl George Simon Jr, Joy P Dunkers
Abstract: Nanofiber technology has emerged as a promising tool to recapitulate the native extracellular matrix (ECM) environment for tissue engineering and regenerative medicine strategies. Cell-material interactions in the nanofiber system are largely de ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914988

9. Structural Basis for Inactivation of Giardia lamblia Carbamate Kinase by Disulfiram
Topic: Bioscience & Health
Published: 4/11/2014
Authors: Andrey Galkin, Liudmila Kulakova, Kap Lim, Catherine Chen, Wei Zhang, Illarion V Turko, Osnat Herzberg
Abstract: Carbamate kinase from Giardia lamblia is an essential enzyme for the survival of the organism. The enzyme catalyzes the final step in the arginine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate. We previously repo ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915235

10. Measuring Stem Cell Dimensionality in Tissue Scaffolds
Topic: Bioscience & Health
Published: 4/1/2014
Authors: Carl George Simon Jr, Sapun Parekh, Christopher K. Tison, Girish Kumar, Tanya M. Farooque, Charles H Camp
Abstract: Many 3D scaffold systems have evolved for tissue engineering, drug screening and in vitro tissue models. However, it is not clear which scaffolds provide a 3D cell niche and there is no clear way to measure cell niche dimensionality. Advances in 3D ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913148



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