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Author: Gregory Cooksey
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1. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Michael W Halter, Elianna Bier, Paul C DeRose, Gregory A Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...
2. Pneumatic valves in folded 2-D and 3-D fluidic devices made from plastic sheets and tapes
Gregory A Cooksey, Francisco Javier Atencia
We present a rapid prototyping technique that expands elastomeric valving capabilities to devices
made from thin materials such as plastic films and tapes. A key characteristic of this technology is
that devices are thin (typically less than 0.5 ...
3. Measurement and validation of cell-based assays with microfluidics at the
National Institute of Standards and Technology (NIST)
Gregory A Cooksey, Francisco Javier Atencia, Samuel P Forry
4. A robust diffusion-based gradient generator for dynamic cell assays.
Francisco Javier Atencia, Gregory A Cooksey, Laurie E Locascio
This manuscript describes a new method to generate purely diffusive chemical gradients that can be modified in time. The device
is simple in its design and easy to use, which makes it amenable to study biological processes that involve static or dy ...
5. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Gregory A Cooksey, John T Elliott, Anne L Plant
Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content
cell-based assays. However, maximizing the value of these assays for drug discovery, screening or
toxicity evaluation, for example, will require va ...
6. Chips and Tips: Modified luer adapters with small dead volume that fit small tubing
Gregory A Cooksey, glynnis Mattheisen
Luer adapters are convenient tools to rapidly and reversibly connect tubing from fluid reservoirs to microfluidic devices. One problem associated with the use of these adapters is that they trap considerable dead volume (60 ul, in the case of the ada ...
7. Magnetic connectors for microfluidic applications
Francisco Javier Atencia, Gregory A Cooksey, Andreas Jahn, Justin M Zook, Wyatt N Vreeland, Laurie E Locascio
We present a new type of connector based on a simple idea: using a ring magnet on one side of the microfluidic chip and a disc magnet on the other side to provide the interfacial force necessary to provide a sealed connection between tubing or needle ...
8. Controlling the Flow Rate of Many Fluids With Floating Vials In a Pressurizeable Container
Gregory A Cooksey, Anne L Plant
We report a simple strategy to control the pressure-driven flow rates from fluid reservoirs to a microchip in an efficient and compact fashion. We demonstrate that using a floating reservoir, less than 1 percent change in flow rate per ml of deliver ...
9. A vacuum manifold for rapid world-to-chip connectivity of complex PDMS microdevices
Gregory A Cooksey, Anne L Plant, Francisco Javier Atencia
The lack of simple interfaces for microfluidic devices with a large number of inlets significantly limits production and utilization of these devices. In this paper, we describe the fabrication of a reusable manifold that provides rapid world-to-chi ...