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You searched on: Author: Michael Halter

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1. Dimensional Metrology of Lab on a Chip Internal Structures: a Comparison of Optical Coherence Tomography with Coherence Fluorescence Microscopy
Published: 4/8/2015
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: The characterization of internal structures in a polymeric device, specifically of a final product, will require a different set of metrology techniques than those traditionally use in the characterization of microelectronic devices. OCT is relati ...

2. High Resolution Surface Plasmon Resonance Imaging for Single Cells
Published: 12/1/2014
Authors: Alexander W Peterson, Michael W Halter, Alessandro Tona, Anne L Plant
Abstract: Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However, c ...

3. Nonparametric Estimates of Drift and Diffusion Profiles via Fokker-Planck Algebra
Published: 10/13/2014
Authors: Steven P Lund, Michael W Halter, Joseph Bunton Hubbard
Abstract: Diffusion processes superimposed upon deterministic motion play a key role in understanding and controlling the transport of matter, energy, momentum, and even information in physics, chemistry, material science, biology, and communications techn ...

4. Automated Ranking of Stem Cell Colonies by Translating Biological Rules to Computational Models
Published: 9/20/2014
Authors: Adele P Peskin, Steven P Lund, YaShian Li-Baboud, Michael W Halter, Anne L Plant, Peter Bajcsy
Abstract: This paper addresses the problem of automating an image ranking process for stem cell colonies. We automate the manual process in a novel way: instead of fitting off-the-shelf image features and colony ranks to prediction models, we define a new feat ...

5. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Published: 8/11/2014
Authors: Michael W Halter, Elianna Bier, Paul C DeRose, Gregory A Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Abstract: Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...

6. Testing Equality of Cell Populations Based on Shape and Geodesic Distance
Published: 12/3/2013
Authors: Robert Charles Hagwood, Javier Bernal, Michael W Halter, John T Elliott
Abstract: Image cytometry has emerged as a valuable in-vitro screening tool and advances in automated microscopy have made it possible to readily analyze large cellular populations of image data. A statistical procedure to study homogeneity of such data ...

7. Optical Coherence Tomography for Dimensional Metrology of Lab-on-a-chip devices
Published: 10/27/2013
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: Here we present a new metrology tool for the determination of the dimensions of channels from final product Lab-on-a-chip devices. Using Optical Coherence Tomography (OCT) areas of up to 12 mm x 12 mm can be imaged in less than 1 second, without the ...

8. Measurement Uncertainty in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7954
Published: 8/13/2013
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Abstract: Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance ...

9. Boltzmann's H-Function and Diffusion Processes
Published: 5/15/2013
Authors: Steven P Lund, Joseph Bunton Hubbard, Michael W Halter
Abstract: There exists a generalization of Boltzmann's $H$-function that allows for non-uniformly populated stationary states which may exist far from thermodynamic equilibrium. Here we describe a method for obtaining a generalized or collective diffusion coef ...

10. Metrology for Lab-on-a-Chip Final-Product Devices
Published: 5/12/2013
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: New metrology tools to measure the critical parameters of internal structures in lab on a chip devices are greatly needed in order to develop standard tests for this technology. Here we present a method that combines a custom made optical coherence ...

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