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You searched on: Author: Karen Phinney

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1. Separation of monosaccharides hydrolyzed from glycoproteins without the need for derivatization
Published: 5/18/2015
Authors: Mark S Lowenthal, Eric L Kilpatrick, Karen W Phinney
Abstract: Chromatographic separation of monosaccharides hydrolyzed from glycoconjugates or complex, aggregate biomaterials, can be achieved by classic analytical methods without a need for derivatizing the monosaccharide subunits. A simple and sensitive metho ...

2. Quantitative performance of internal standard platforms for absolute protein quantification using MRM-MS
Published: 3/26/2015
Authors: Kerry M Bauer, Illarion V Turko, Karen W Phinney
Abstract: Isotope dilution mass spectrometry involves the addition of known quantities of isotope-labeled standards, that mimic native molecules, to biological samples. We evaluated three conventional internal standard platforms (synthetic peptides, QconCAT co ...

3. Current Trends in Magnetic Particle Enrichment for Mass Spectrometry-Based Analysis of Cardiovascular Protein Biomarkers
Published: 2/18/2015
Authors: Nicole A. Schneck, Mark S Lowenthal, Karen W Phinney, Sang Bok Lee
Abstract: Magnetic particles have traditionally been utilized to isolate and enrich various cardiovascular protein biomarkers for mass spectrometry-based proteomic analysis. The application of functionalized magnetic particles for immunocapture is attracti ...

4. An LC-MS/MS method for serum methylmalonic acid suitable for monitoring vitamin B12 status in population surveys compares well to the previous NHANES GC/MS method and to the NIST reference measurement procedure
Published: 9/26/2014
Authors: Karen W Phinney, Ekaterina M. Mineva, Mindy Zhang, Daniel Rabinowitz, Christine M Pfeiffer

5. Analysis of Deamidation Artifacts Induced by Microwave-Assisted Tryptic Digestion of a Monoclonal Antibody
Published: 7/31/2014
Authors: Catherine A Formolo, Nathanael A Heckert, Karen W Phinney
Abstract: The thorough characterization of biopharmaceuticals is essential for ensuring their quality and safety, since many potential variations can cause changes to a drug‰s properties that may be detrimental to the patient. Prior to approval and release, p ...

6. Multiplexed LC-MS/MS Assay for Urine Albumin
Published: 7/24/2014
Authors: Ashley Beasley Green, David M Bunk, Karen W Phinney
Abstract: Targeted Multiplexed Assay for Urinary Albumin Quantification Ashley Beasley-Green, Nijah M. Burris, David Bunk, Karen Phinney Biomolecular Measurement Division National Institute of Standards and Technology, Gaithersburg, MD ABSTRACT Uri ...

7. Assessing Vitamin D Status: Time for a Rethink?
Published: 2/19/2014
Authors: Karen W Phinney, Graham D. Carter

8. Quantitative bottom-up proteomics depends on digestion conditions
Published: 12/2/2013
Authors: Mark S Lowenthal, Yuxue Liang, Karen W Phinney, Stephen E Stein
Abstract: Accurate quantification is a fundamental requirement in the fields of proteomics and biomarker discovery, and for clinical diagnostic assays. To demonstrate the extent of quantitative variability in measurable peptide concentrations due to differenc ...

9. Development of a Standard Reference Material for Metabolomics Research
Published: 11/4/2013
Authors: Karen W Phinney, Guillaume Ballihaut, Mary Bedner, Johanna Camara, Steven J Christopher, William C Davis, Nathan G. Dodder, Brian E Lang, Stephen E Long, Mark S Lowenthal, Elizabeth A. McGaw, Karen E Murphy, Bryant C Nelson, Jocelyn L. Prendergast, Jessica L Reiner, Catherine A Rimmer, Lane C Sander, Michele Miller Schantz, Katherine E Sharpless, Lorna Tregoning Sniegoski, Susan Shu Cheng Tai, Jeanice M Brown Thomas, Thomas W Vetter, Michael James Welch, Stephen A Wise, Laura J Wood, William F Guthrie, Robert Charles Hagwood, Stefan D Leigh, James H Yen, Nien F Zhang, Madhu Chaurhary-Webb, Huiping Chen, Bridgette Haynes, Donna J LaVoie, Leslie F McCoy, Shahzad S Momin, Neelima Paladugula, Elizabeth C Pendergrast, Christine M Pfeiffer, Carissa D Powers, Zia Fazili-Qari, Daniel Rabinowitz, Michael E Rybak, Rosemary L Schleicher, Mary Xu, Mindy Zhang, Arthur L Castle, Brandi S Benford, Gauthier Eppe

10. Metabolite Profiling of a NIST Standard Reference Material for Human Plasma (SRM 1950) ‹ GC/MS, LC/MS, NMR and Clinical Laboratory Analyses, Libraries and Web-based resources
Published: 10/22/2013
Authors: Yamil Simon, Mark S Lowenthal, Lisa Ervin Kilpatrick, Maureen L Sampson, Kelly H Telu, Paul A Rudnick, William Gary Mallard, Daniel W Bearden, Tracey Beth Schock Schock, Dmitrii V Tchekhovskoi, Niksa Blonder, Xinjian Yan, Yuxue Liang, Yufang Zheng, William E Wallace III, Pedatsur Neta, Karen W Phinney, Alan T. Remaley, Stephen E Stein
Abstract: Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest in global profiling, i.e., semi-quantitative monitoring of all chemical constituents of biological fluids. In this work, we have ...

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