You searched on: Author: Karen Phinney
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1. Quantitative performance of internal standard platforms for absolute protein quantification using MRM-MS
Kerry M Bauer, Illarion V Turko, Karen W Phinney
Isotope dilution mass spectrometry involves the addition of known quantities of isotope-labeled standards, that mimic native molecules, to biological samples. We evaluated three conventional internal standard platforms (synthetic peptides, QconCAT co ...
2. Current Trends in Magnetic Particle Enrichment for Mass Spectrometry-Based Analysis of Cardiovascular
Nicole A. Schneck, Mark S Lowenthal, Karen W Phinney, Sang Bok Lee
Magnetic particles have traditionally been utilized to isolate and enrich various cardiovascular
protein biomarkers for mass spectrometry-based proteomic analysis. The application of functionalized
magnetic particles for immunocapture is attracti ...
3. An LC-MS/MS method for serum methylmalonic acid suitable for monitoring vitamin B12 status in population surveys compares well to the previous NHANES GC/MS method and to the NIST reference measurement procedure
Karen W Phinney, Ekaterina M. Mineva, Mindy Zhang, Daniel Rabinowitz, Christine M Pfeiffer
4. Analysis of Deamidation Artifacts Induced by Microwave-Assisted Tryptic Digestion of a Monoclonal Antibody
Catherine A Formolo, Nathanael A Heckert, Karen W Phinney
The thorough characterization of biopharmaceuticals is essential for ensuring their quality and safety, since many potential variations can cause changes to a drug‰s properties that may be detrimental to the patient. Prior to approval and release, p ...
5. Multiplexed LC-MS/MS Assay for Urine Albumin
Ashley Beasley Green, David M Bunk, Karen W Phinney
Targeted Multiplexed Assay for Urinary Albumin Quantification
Ashley Beasley-Green, Nijah M. Burris, David Bunk, Karen Phinney
Biomolecular Measurement Division
National Institute of Standards and Technology, Gaithersburg, MD
6. Assessing Vitamin D Status: Time for a Rethink?
Karen W Phinney, Graham D. Carter
7. Quantitative bottom-up proteomics depends on digestion conditions
Mark S Lowenthal, Yuxue Liang, Karen W Phinney, Stephen E Stein
Accurate quantification is a fundamental requirement in the fields of proteomics and biomarker discovery, and for clinical diagnostic assays. To demonstrate the extent of quantitative variability in measurable peptide concentrations due to differenc ...
8. Development of a Standard Reference Material for Metabolomics Research
Karen W Phinney, Guillaume Ballihaut, Mary Bedner, Johanna Camara, Steven J Christopher, William C Davis, Nathan G. Dodder, Brian E Lang, Stephen E Long, Mark S Lowenthal, Elizabeth A. McGaw, Karen E Murphy, Bryant C Nelson, Jocelyn L. Prendergast, Jessica L Reiner, Catherine A Rimmer, Lane C Sander, Michele M Schantz, Katherine E Sharpless, Lorna Tregoning Sniegoski, Susan Shu Cheng Tai, Jeanice M Brown Thomas, Thomas W Vetter, Michael James Welch, Stephen A Wise, Laura J Wood, William F Guthrie, Robert Charles Hagwood, Stefan D Leigh, James H Yen, Nien F Zhang, Madhu Chaurhary-Webb, Huiping Chen, Bridgette Haynes, Donna J LaVoie, Leslie F McCoy, Shahzad S Momin, Neelima Paladugula, Elizabeth C Pendergrast, Christine M Pfeiffer, Carissa D Powers, Zia Fazili-Qari, Daniel Rabinowitz, Michael E Rybak, Rosemary L Schleicher, Mary Xu, Mindy Zhang, Arthur L Castle, Brandi S Benford, Gauthier Eppe
9. Metabolite Profiling of a NIST Standard Reference Material for Human Plasma (SRM 1950) ‹ GC/MS, LC/MS, NMR and Clinical Laboratory Analyses, Libraries and Web-based resources
Yamil Simon, Mark S Lowenthal, Lisa Ervin Kilpatrick, Maureen L Sampson, Kelly H Telu, Paul A Rudnick, William Gary Mallard, Daniel W Bearden, Tracey Beth Schock Schock, Dmitrii V Tchekhovskoi, Niksa Blonder, Xinjian Yan, Yuxue Liang, Yufang Zheng, William E Wallace III, Pedatsur Neta, Karen W Phinney, Alan T. Remaley, Stephen E Stein
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest in global profiling, i.e., semi-quantitative monitoring of all chemical constituents of biological fluids. In this work, we have ...
10. Quantification of transferrin in human serum using both QconCAT and synthetic internal standards
Tyler A. (Tyler) Zimmerman, Meiyao M. Wang, Mark S Lowenthal, Illarion V Turko, Karen W Phinney
Transferrin, an iron transport protein, is a clinically-important biomarker in diseases such as
iron-deficiency anemia. Current diagnostic methods for transferrin levels lack quantitative
accuracy, suggesting the need for alternative approaches ...