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You searched on: Author: Karen Phinney

Displaying records 1 to 10 of 61 records.
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1. Quantification of Borrelia burgdorferi membrane proteins in human serum: a new concept for detection of bacterial infection
Published: 11/17/2015
Authors: Sao F. Cheung, Kyle W Anderson, Kenia Benitez, Mark J Soloski, John N Aucott, Karen W Phinney, Illarion V Turko
Abstract: The low abundance of bacterial proteins in human serum upon infection imposes a challenge for the early proteomic detection of bacterial infection. To address this challenge, we propose to take advantage of detecting membrane proteins released from b ...

2. Standardizing 25-Hydroxyvitamin D values from the Canadian Health Measures Survey
Published: 9/30/2015
Authors: Kurtis Sarafin, Ramon Durazo-Arvizu, Lu Tian, Karen W Phinney, Susan Shu Cheng Tai, Johanna Camara, Hubert W. Vesper, Joyce M. Merkel, Evan Green, Christopher T. Sempos, Stephen PJ Brooks
Abstract: Background: The Canadian Health Measures survey (CHMS) is an ongoing cross sectional national survey that includes a measure of 25-hydroxyvitamin D (25(OH)D) by Diasorin LIAISON immunoassay. The survey repeats approximately every two years with a ne ...

3. Separation of monosaccharides hydrolyzed from glycoproteins without the need for derivatization
Published: 5/18/2015
Authors: Mark S Lowenthal, Eric L Kilpatrick, Karen W Phinney
Abstract: Chromatographic separation of monosaccharides hydrolyzed from glycoconjugates or complex, aggregate biomaterials, can be achieved by classic analytical methods without a need for derivatizing the monosaccharide subunits. A simple and sensitive metho ...

4. Quantitative performance of internal standard platforms for absolute protein quantification using MRM-MS
Published: 3/26/2015
Authors: Kerry M Bauer, Illarion V Turko, Karen W Phinney
Abstract: Isotope dilution mass spectrometry involves the addition of known quantities of isotope-labeled standards, that mimic native molecules, to biological samples. We evaluated three conventional internal standard platforms (synthetic peptides, QconCAT co ...

5. Current Trends in Magnetic Particle Enrichment for Mass Spectrometry-Based Analysis of Cardiovascular Protein Biomarkers
Published: 2/18/2015
Authors: Nicole Ashley Schneck, Mark S Lowenthal, Karen W Phinney, Sang Bok Lee
Abstract: Magnetic particles have traditionally been utilized to isolate and enrich various cardiovascular protein biomarkers for mass spectrometry-based proteomic analysis. The application of functionalized magnetic particles for immunocapture is attracti ...

6. An LC-MS/MS method for serum methylmalonic acid suitable for monitoring vitamin B12 status in population surveys compares well to the previous NHANES GC/MS method and to the NIST reference measurement procedure
Published: 9/26/2014
Authors: Karen W Phinney, Ekaterina M. Mineva, Mindy Zhang, Daniel Rabinowitz, Christine M Pfeiffer

7. Analysis of Deamidation Artifacts Induced by Microwave-Assisted Tryptic Digestion of a Monoclonal Antibody
Published: 7/31/2014
Authors: Catherine A Formolo, Nathanael A Heckert, Karen W Phinney
Abstract: The thorough characterization of biopharmaceuticals is essential for ensuring their quality and safety, since many potential variations can cause changes to a drug‰s properties that may be detrimental to the patient. Prior to approval and release, p ...

8. Multiplexed LC-MS/MS Assay for Urine Albumin
Published: 7/24/2014
Authors: Ashley Beasley Green, David M Bunk, Karen W Phinney
Abstract: Targeted Multiplexed Assay for Urinary Albumin Quantification Ashley Beasley-Green, Nijah M. Burris, David Bunk, Karen Phinney Biomolecular Measurement Division National Institute of Standards and Technology, Gaithersburg, MD ABSTRACT Uri ...

9. Assessing Vitamin D Status: Time for a Rethink?
Published: 2/19/2014
Authors: Karen W Phinney, Graham D. Carter

10. Quantitative bottom-up proteomics depends on digestion conditions
Published: 12/2/2013
Authors: Mark S Lowenthal, Yuxue Liang, Karen W Phinney, Stephen E Stein
Abstract: Accurate quantification is a fundamental requirement in the fields of proteomics and biomarker discovery, and for clinical diagnostic assays. To demonstrate the extent of quantitative variability in measurable peptide concentrations due to differenc ...

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  • SP 250-XX: Calibration Services
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