You searched on: Author: John Elliott
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1. Strategies for Achieving Measurement Assurance for Cell Therapy Products
Carl George Simon Jr., Anne L Plant, Sumona Sarkar, John T Elliott, Sheng Lin-Gibson
Cell therapy products (CTPs) need quantitative, validated, and robust assays to support
informed decision making during their development, manufacturing, and regulation. These
products, the materials involved in their production and the methods f ...
2. Reliable preparation of nanoparticle agglomerates of different sizes in cell culture media
Special Publication (NIST SP)
Justin M Zook, Robert MacCuspie, John T Elliott, Elijah J Petersen
This special publication is one in a series stemming from the National Nanotechnology Initiative
(NNI) Nano-EHS Research Strategy which identified Nanomaterial Measurement Infrastructure as one of
the essential areas of research needed in order t ...
3. The Use of Cause-and-Effect Analysis to Design a High Quality Nano-Cyto-Toxicology Assay
Matthias Rosslein, John T Elliott, Marc L Salit, Elijah J Petersen, Cordula Hirsch, Harald Krug, Peter Wick
An important consideration in developing standards and regulations that govern the production and
use of commercial nanoscale materials is the development of robust and reliable measurements to
assess potential biological effects of these nanomat ...
4. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Michael W Halter, Elianna Bier, Paul C DeRose, Gregory Alan Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...
5. Highly Stable Positively Charged Dendron-Encapsulated Gold Nanoparticles
Tae Joon Cho, Robert I. MacCuspie, Julien C. Gigault, Justin M Gorham, John T Elliott, Vincent A Hackley
We report the development of a novel cationic dendron (TAG1-PCD) and a positively charged gold nanoparticle-dendron conjugate (PCD-AuNP). TAG1-PCD was designed by considering the reactivity, hydrophilicity, and cationic nature that is required to yie ...
6. Testing Equality of Cell Populations Based on Shape and Geodesic Distance
Robert Charles Hagwood, Javier Bernal, Michael W Halter, John T Elliott
Image cytometry has emerged as a valuable in-vitro screening tool and advances in automated microscopy have made it possible to readily analyze large cellular populations of image data. A statistical procedure to study homogeneity of such data ...
7. Estimating the Number of Manually Segmented Cellular Objects Required to Evaluate the Accuracy of a Segmentation Algorithm
Adele P Peskin, Joe Chalfoun, John T Elliott, Karen Kafadar
We propose a new strategy for estimating the number of cellular objects that should be manually segmented for evaluating the segmentation accuracy of an algorithm. The strategy uses geometric and edge quality measurements that are directly related to ...
8. Measurement Uncertainty in Cell Image Segmentation Data Analysis
NIST Interagency/Internal Report (NISTIR)
Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior,
and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is
fundamentally important to evaluate the performance ...
9. Understanding impact of image quality on segmentation accuracy
YaShian Li-Baboud, Antonio Cardone, Joe Chalfoun, Peter Bajcsy, John T Elliott
We introduce a methodology for microscopy settings assessment and segmentation accuracy prediction based on image quality descriptors.
10. NIST Gold Nanoparticle Reference Materials Do Not Induce Oxidative DNA Damage
Bryant C Nelson, Donald H Atha, John T Elliott, Bryce J. Marquis, Elijah J Petersen, Danielle Cleveland, Stephanie S Watson, I-Hsiang Tseng, Andrew Dillon, Melissa Theodore, Joany Jackman
Well-characterized, nanoparticle reference materials are urgently needed for nanomaterial toxicity studies. The National Institute of Standards and Technology has developed three gold nanoparticle (AuNP) reference materials (10 nm, 30 nm, 60 nm) to ...