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Author: John Elliott

Displaying records 1 to 10 of 43 records.
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1. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Published: 8/11/2014
Authors: Michael W Halter, Elianna Bier, Paul C DeRose, Gregory A Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Abstract: Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915188

2. Highly Stable Positively Charged Dendron-Encapsulated Gold Nanoparticles
Published: 3/13/2014
Authors: Tae Joon Cho, Robert I. MacCuspie, Julien C. Gigault, Justin M Gorham, John T Elliott, Vincent A Hackley
Abstract: We report the development of a novel cationic dendron (TAG1-PCD) and a positively charged gold nanoparticle-dendron conjugate (PCD-AuNP). TAG1-PCD was designed by considering the reactivity, hydrophilicity, and cationic nature that is required to yie ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914562

3. Testing Equality of Cell Populations Based on Shape and Geodesic Distance
Published: 12/3/2013
Authors: Robert Charles Hagwood, Javier Bernal, Michael W Halter, John T Elliott
Abstract: Image cytometry has emerged as a valuable in-vitro screening tool and advances in automated microscopy have made it possible to readily analyze large cellular populations of image data. A statistical procedure to study homogeneity of such data ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911573

4. Estimating the Number of Manually Segmented Cellular Objects Required to Evaluate the Accuracy of a Segmentation Algorithm
Published: 9/22/2013
Authors: Adele P Peskin, Joe Chalfoun, John T Elliott, Karen Kafadar
Abstract: We propose a new strategy for estimating the number of cellular objects that should be manually segmented for evaluating the segmentation accuracy of an algorithm. The strategy uses geometric and edge quality measurements that are directly related to ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913694

5. Measurement Uncertainty in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7954
Published: 8/13/2013
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Abstract: Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914171

6. Understanding impact of image quality on segmentation accuracy
Published: 8/6/2013
Authors: YaShian Li-Baboud, Antonio Cardone, Joe Chalfoun, Peter Bajcsy, John T Elliott
Abstract: We introduce a methodology for microscopy settings assessment and segmentation accuracy prediction based on image quality descriptors.
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913788

7. NIST Gold Nanoparticle Reference Materials Do Not Induce Oxidative DNA Damage
Published: 2/1/2013
Authors: Bryant C Nelson, Donald H Atha, John T Elliott, Bryce J. Marquis, Elijah J Petersen, Danielle Cleveland, Stephanie S Watson, I-Hsiang Tseng, Andrew Dillon, Melissa Theodore, Joany Jackman
Abstract: Well-characterized, nanoparticle reference materials are urgently needed for nanomaterial toxicity studies. The National Institute of Standards and Technology has developed three gold nanoparticle (AuNP) reference materials (10 nm, 30 nm, 60 nm) to ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906893

8. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

9. New Concepts for Building Vocabulary for Cell Image Ontologies
Published: 12/21/2011
Authors: Anne L Plant, John T Elliott, Talapady N Bhat
Abstract: Background: We present an approach to cell image databasing that is compatible with searching across a global federation of independent image databases. The variety of biological experiments and data that practitioners would like to access and share ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907774

10. Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
Published: 12/1/2011
Authors: Justin M Zook, Robert I. MacCuspie, Laurie E Locascio, Michael W Halter, John T Elliott
Abstract: Methods to disperse nanoparticles reproducibly in aqueous solutions compatible with cell-based assays are critical for studying cytotoxicity. Here, we disperse gold and silver nanoparticles in cell culture media, letting them agglomerate to a variet ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906154



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