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You searched on: Author: Kenneth Cole

Displaying records 1 to 10 of 49 records.
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1. Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry
Published: 12/11/2014
Authors: Meiyao M. Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Jeffrey M. Davis, Kenneth D Cole, Illarion V Turko, Lili Wang, Fatima Abbasi
Abstract: For quantitative flow cytometry, a biological cell reference material with a known biomarker expression level is needed to transform a linear arbitrary fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per c ...

2. Mouse Cell Line Authentication
Published: 2/13/2013
Authors: Jamie L Almeida, Carolyn R Steffen, Kenneth D Cole
Abstract: The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed a multiplex poly ...

3. Breast Cancer Biomarker Measurements and Standards
Published: 1/24/2013
Authors: Kenneth D Cole, Lili Wang, Hua-Jun He
Abstract: Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that ...

4. Authentication of African green monkey cell lines using human short tandem repeat markers
Published: 11/7/2011
Authors: Jamie L Almeida, Carolyn R Steffen, Kenneth D Cole
Abstract: A multiplex PCR assay utilizing eight human short tandem repeat (STR) markers was developed for the authentication of vervet monkey cell lines. Labeled forward primers were used to amplify the targeted STR marker region and amplified products were s ...

5. Electrochemical Inactivation of Bacillus Spores in Water Using a Quaternary Oxide Electrode
Published: 11/1/2011
Authors: Vytautas Reipa, Andrea Giovannozzi, Jamie L Almeida, Kenneth D Cole
Abstract: Bacillus thuringiensis (BT) spores and B. anthracis Sterne (BA) spores were used to investigate the effectiveness of electrochemical disinfection processes using different solution and operational conditions. We tested several anode materials for di ...

6. Validation of Serum Cardiac Troponin I Reference Materials Using an Immunoprecipitation Method Coupled with Fluorescent Western Blot Analysis
Published: 1/14/2011
Authors: Hua-Jun He, Mark S Lowenthal, Kenneth D Cole, David M Bunk, Lili Wang
Abstract: Cardiac troponin I (cTnI) is adopted as the gold standard cardiac biomarker following the universal definition of myocardial infarction (MI). Presently, the performance of commercial cTnI immunoassays varies significantly despite the recalibration ...

7. Development of a Candidate Secondary Reference Procedure (immunoassay based measurement procedure of higher metrological order) for Cardiac Troponin I: I. Antibody Characterization and Preliminary Validation
Published: 11/1/2010
Authors: James E Noble, David M Bunk, R. H. Christenson, Kenneth D Cole, Hua-Jun He, Alexei Katrukha, Mauro Panteghini, Robert Porter, Heinz Schimmel, Jillian Tate, Lili Wang
Abstract: The development of a reference measurement procedure to support the cardiac troponin I (cTnI) standardization initiative is described. The reference measurement procedure will be used to assign reference values to serum-based secondary standards. A m ...

8. Building Plumbing System Decontamination - First Report on Recommendations
Series: Technical Note (NIST TN)
Report Number: 1652
Published: 9/6/2009
Authors: Stephen J. Treado, Mark A Kedzierski, Stephanie S Watson, Nicos Martys, Kenneth D Cole
Abstract: This report summarizes the measurement results and recommended procedures for responding to building plumbing system contamination incidents and restoring the water system to safe operation. The recommendations are based on analysis of the results o ...

9. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...

10. Contaminant Accumulation and Removal in Building Plumbing Systems
Series: Technical Note (NIST TN)
Report Number: 1605
Published: 9/30/2008
Authors: Stephen J. Treado, Mark A Kedzierski, Daniel Robert Flynn, David Wilmering, Thomas D Montgomery, Kenneth D Cole
Abstract: This report summarizes measurements and results conducted to evaluate the accumulation and removal of water-borne contaminants in building plumbing systems. In particular, tests were conducted at the BFRL Plumbing Test Facility, which emulates a ful ...

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