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Author: Kiran Bhadriraju

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1. Frictional properties of native and functionalized type I collagen thin films
Published: 10/2/2013
Authors: Koo-hyun Chung, Antony Chen, Christopher Anderton, Kiran Bhadriraju, Anne L Plant, Brian G Bush, Robert Francis Cook, Frank W DelRio
Abstract: Frictional properties of native and fibronectin (FN)-functionalized type I collagen (COL) thin films were studied via atomic force microscopy. The COL lateral contact stiffness was dependent only on the hydration state, indicating that shear deformat ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914307

2. Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins
Published: 4/22/2013
Authors: Antony K. Chen, Frank W DelRio, Alexander W Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L Plant
Abstract: The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910923

3. ELUCIDATING THE SURFACE COMPOSITION OF MECHANICALLY MODULATED COLLAGEN FIBRIL FILMS WITH TOF-SIMS
Published: 1/17/2013
Authors: Christopher R Anderton, Kiran Bhadriraju, Frank W DelRio, Anne L Plant
Abstract: The extracellular matrix (ECM) environment plays a critical role in organism development and disease. Consequently, developing complementary analytical techniques to study bioactive scaffolds can assist in understanding cell-ECM processes. We have ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910931

4. Biological Cell Feature Identification by a Modified Watershed-Merging Algorithm
Series: NIST Interagency/Internal Report (NISTIR)
Published: 11/24/2010
Authors: David E. Gilsinn, Kiran Bhadriraju, John T Elliott
Abstract: Biological cells are composed of many subsystems and organelles. The subsystem called the cytoskeleton is composed of long rod-shaped structures. They give the cell form and help attach the cell to the substrate and neighbors. One of the filaments is ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906615

5. Image Classification of Vascular Smooth Muscle Cells
Published: 11/11/2010
Authors: Michael Grasso, Ronil Mokashi , Alden A Dima, Antonio Cardone, Kiran Bhadriraju, Anne L Plant, Mary C Brady, Yaacov Yesha, Yelena Yesha
Abstract: The traditional method of cell microscopy can be subjective, due to observer variability, a lack of standardization, and a limited feature set. To address this challenge, we developed an image classifier using a machine learning approach. Our system ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905981

6. Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix
Published: 7/13/2010
Authors: Alexander W Peterson, Michael W Halter, Alessandro Tona, Kiran Bhadriraju, Anne L Plant
Abstract: Details of cellular interaction with a fibronectin coated surface were examined using surface plasmon resonance imaging (SPRI). SPRI is a label-free highly sensitive technique that is based on the measurement of interfacial refractive index. SPRI is ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903817

7. The Nanomechanical Properties of Thin Film of Type I Collagen Fibrils
Published: 3/2/2010
Authors: Koo-Hyun Chung, Kiran Bhadriraju, Tighe Spurlin, Robert Francis Cook, Anne L Plant
Abstract: Cells sense and respond to the mechanical properties of the substrate to which they adhere. Matrices composed of collagen have been an important experimental system to study cell responses to the stiffness of the extracellular matrix. We had previo ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=824709

8. Surface plasmon resonance imaging of cells and surface-associated fibronectin
Published: 2/26/2009
Authors: Alexander W Peterson, Michael W Halter, Alessandro Tona, Kiran Bhadriraju, Anne L Plant
Abstract: Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=902594

9. The Stiffness of Collagen Fibrils Influences Vascular Smooth Muscle Cell Phenotype
Published: 3/1/2007
Authors: Dennis P. McDaniel, Gordon Allan Shaw, John T Elliott, Kiran Bhadriraju, Curtis W Meuse, Koo-Hyun Chung, Anne L Plant
Abstract: Cells receive signals from the extracellular matrix through receptor-dependent interactions, but they are also influenced by the mechanical properties of the matrix. While bulk properties of substrates have been shown to effect cell behavior, we sho ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830514



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