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You searched on: Author: peter vallone

Displaying records 31 to 40 of 41 records.
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31. A Strategy for Examining Complex Mixtures of Deoxyoligonucleotides Using Ion-Pair Reverse-Phase High-Performance Liquid Chromatography, Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, and Informatics
Published: 5/15/2002
Authors: Peter M Vallone, J. M. Devaney, M A Marino, John M Butler

32. A Strategy for Examining Complex Mixtures of Deoxyoligonucleotides Using IP-RP HPLC, MALDI-TOF MD, and Informatics
Published: 3/17/2002
Authors: Peter M Vallone, J E Devaney, J M Marino
Abstract: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)and ion pair-reverse phase high performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the sequence identity of short single-stra ...

33. General Strategy for the Design of Multiplex PCR Assays Involving Y STR Markers
Published: 1/1/2002
Authors: R Schoske, Peter M Vallone, C M. Ruitberg, John M Butler
Abstract: The simultaneous amplification of multiple regions of a DNA template is routinely performed using the Polymerase Chain Reaction (PCR) in a process termed multiplex PCR. A useful strategy involving the design, testing and optimization of multiplex PC ...

34. Development of Y STR Megaplex Assays
Published: 12/1/2001
Authors: R Schoske, John M Butler, Peter M Vallone, Margaret C Kline, M. Prinz, A J Redd, M F Hammer
Abstract: Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. ...

35. Calculating sequence-dependent melting stability of duplex DNA oligomers and multiplex sequence analysis by graphs
Published: 9/1/2001
Authors: A. S. Benight, P. Pancoska, R. Owczarzy, Peter M Vallone, J. Nesetril, P. V. Riccelli

36. Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions
Published: 6/1/2001
Authors: John M Butler, J E Devaney, M A Marino, Peter M Vallone
Abstract: Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplific ...

37. Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions
Published: 2/1/2001
Authors: John M Butler, C M. Ruitberg, Peter M Vallone
Abstract: Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and exam ...

38. DNA Mass Spectrometry at NIST
Published: 2/1/2001
Author: Peter M Vallone
Abstract: The use of mass spectrometry as a tool for solving biochemical problems is growing. Specifically, matrix assisted laser desorption ionization coupled with time-of-flight detection systems (MALDI-TOF) is emerging for high throughput and highaccuracy ...

39. Genotyping of Two Mutations in the HFE Gene Using Single-Base Extension and High-Performance Liquid Chromatography
Published: 2/1/2001
Authors: J. M. Devaney, E. L. Pettit, S. G. Kaler, Peter M Vallone, John M Butler, M. A. Marino

40. Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA
Published: 2/1/2000
Authors: Peter M Vallone, R. Owczarzy, P. V. Riccelli, A. S. Benight
Abstract: Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA. Optical and DSC melting studies were performed on 12 DNAdumbbells in 85 mM Na+. The stem ...

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