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Author: peter vallone

Displaying records 31 to 39.
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31. General Strategy for the Design of Multiplex PCR Assays Involving Y STR Markers
Published: 1/1/2002
Authors: R Schoske, Peter M Vallone, C M. Ruitberg, John M Butler
Abstract: The simultaneous amplification of multiple regions of a DNA template is routinely performed using the Polymerase Chain Reaction (PCR) in a process termed multiplex PCR. A useful strategy involving the design, testing and optimization of multiplex PC ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830282

32. Development of Y STR Megaplex Assays
Published: 12/1/2001
Authors: R Schoske, John M Butler, Peter M Vallone, Margaret C Kline, M. Prinz, A J Redd, M F Hammer
Abstract: Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830279

33. Calculating sequence-dependent melting stability of duplex DNA oligomers and multiplex sequence analysis by graphs
Published: 9/1/2001
Authors: A. S. Benight, P. Pancoska, R. Owczarzy, Peter M Vallone, J. Nesetril, P. V. Riccelli
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903956

34. Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions
Published: 6/1/2001
Authors: John M Butler, J E Devaney, M A Marino, Peter M Vallone
Abstract: Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplific ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830184

35. Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions
Published: 2/1/2001
Authors: John M Butler, C M. Ruitberg, Peter M Vallone
Abstract: Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and exam ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830191

36. DNA Mass Spectrometry at NIST
Published: 2/1/2001
Author: Peter M Vallone
Abstract: The use of mass spectrometry as a tool for solving biochemical problems is growing. Specifically, matrix assisted laser desorption ionization coupled with time-of-flight detection systems (MALDI-TOF) is emerging for high throughput and highaccuracy ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830226

37. Genotyping of Two Mutations in the HFE Gene Using Single-Base Extension and High-Performance Liquid Chromatography
Published: 2/1/2001
Authors: J. M. Devaney, E. L. Pettit, S. G. Kaler, Peter M Vallone, John M Butler, M. A. Marino
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903965

38. Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA
Published: 2/1/2000
Authors: Peter M Vallone, R. Owczarzy, P. V. Riccelli, A. S. Benight
Abstract: Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA. Optical and DSC melting studies were performed on 12 DNAdumbbells in 85 mM Na+. The stem ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830154

39. An Investigation of Discrimination Capacity and the Cause of Null Alleles in Linear Array Mitostrips Using Control Region Sequence Data.
Published: Date unknown
Authors: Michael D Coble, Margaret C Kline, Janette W. Redman, Amy E. Decker, Peter M Vallone, John M Butler
Abstract: Mitochondrial DNA (mtDNA) analysis of forensic evidentiary materials such as degraded bones and shed hairs can provide the forensic scientist with some genetic information especially when highly discriminatory systems, such as nuclear STRs, completel ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830521



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