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1. Reconstitution of 2 nm diameter Silicon Nanoparticles (RM8027) into aqueous solvents
Special Publication (NIST SP)
NIST recently has released a SiNP reference material RM8027 that consists of five hermetically
sealed pre-scored glass ampoules, each containing nominally 1 ml of cyclohexane-stabilized
SiNP‰s suspended in toluene. The suspension contains particl ...
2. The DNA Damaging Potential of Photoactivated P25 Titanium Dioxide Nanoparticles
Elijah J Petersen, Vytautas Reipa, Stephanie S Watson, Deborah L Stanley, Savelas A Rabb, Bryant C Nelson
Titanium dioxide nanoparticles (TiO2 NPs) are found in numerous commercial and personal care products. Thus, it is necessary to understand and characterize their potential environmental health and safety risks. It is well known that photoactivated ...
3. Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy
Steve Y. Rhieu, Aaron A Urbas, Daniel W Bearden, John P Marino, Vytautas Reipa, Katrice A Lippa
Non-invasive and real-time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in-cell nuclear magnetic resonance (NMR)-based method for measuring the intracellular glutathion ...
4. Quantitative measurements of glutathione in yeast cell lysate using 1H NMR
Steve Y. Rhieu, Aaron A Urbas, Katrice A Lippa, Vytautas Reipa
A method for quantifying the level of glutathione (GSH) in yeast cell lysate is described using 1H NMR analysis. For quantification purposes, the 1H resonances corresponding to the Cys βCH2 of GSH were identified as having the fewest overlapping ...
5. In situ electrochemical small-angle neutron scattering (eSANS) for quantitative structure and redox properties of nanoparticles
Vivek M Prabhu, Vytautas Reipa
Rapid growth in nanomaterial applications highlight limitations of available physicochemical characterization methods. An in situ electrochemical small-angle neutron scattering (eSANS) meth-odology was devised that enables direct measurements of nano ...
6. Electrochemical Inactivation of Bacillus Spores in Water Using a Quaternary Oxide Electrode
Vytautas Reipa, Andrea Giovannozzi, Jamie L Almeida, Kenneth D Cole
Bacillus thuringiensis (BT) spores and B. anthracis Sterne (BA) spores were used to investigate the effectiveness of electrochemical disinfection processes using different solution and operational conditions. We tested several anode materials for di ...
7. Physicochemical characterization and in vitro hemolysis evaluation of silver nanoparticles
Vytautas Reipa, Jonghoon Choi, Nam Sun Wang, Victoria M. Hitchins, Peter L. Goering, Robert Malinauskas
In anticipation of the increased use of various forms of silver as an antimicrobial agent in medical devices, the objective of this study was to evaluate the in vitro hemolytic potential of silver nanoparticles in dilute human blood, and to relate pa ...
8. Nanomaterial Toxicity: Emerging Standards and Efforts to Support Standards Development
Laurie E Locascio, Vytautas Reipa, Justin M Zook, Richard C. Pleus
For the first time in the history of industrialization, nanotechnology offers the unique opportunity to consider material safety ahead of its full implementation. Many scientists around the world have been motivated by this and are working on develo ...
9. Measurement of Nanoparticle Concentration Using Quartz Crystal Microgravimetry
Vytautas Reipa, Geoffrey Purdum, Jonghoon Choi
A common issue, encountered in nanoparticle-biological system interaction studies is accurate nanoparticle concentration expression, given the critical importance of the dose-response relationships. We present here the application of quartz crystal m ...
10. Association and Redox Properties of the Putidaredoxin Reductase - Oxidized Nicotinamide Adenine Dinucleotide Complex
Vytautas Reipa, Marcia J Holden, V L. Vilker
Putidaredoxin reductase (PdR) is the flavin protein that carries out the first electron transfer involved in the cytochrome P450cam catalytic cycle. In PdR, the oxidized flavin adenine dinucleotide (FAD/FADH2) redox center is reduced by two-electron ...