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1. High Resolution Surface Plasmon Resonance Imaging for Single Cells
Alexander W Peterson, Michael W Halter, Alessandro Tona, Anne L Plant
Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However, c ...
2. Automated Ranking of Stem Cell Colonies by Translating Biological Rules to Computational Models
Adele P Peskin, Steven P Lund, YaShian Li-Baboud, Michael W Halter, Anne L Plant, Peter Bajcsy
This paper addresses the problem of automating an image ranking process for stem cell colonies. We automate the manual process in a novel way: instead of fitting off-the-shelf image features and colony ranks to prediction models, we define a new feat ...
3. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Michael W Halter, Elianna Bier, Paul C DeRose, Gregory A Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...
4. Frictional properties of native and functionalized type I collagen thin films
Koo-hyun Chung, Antony Chen, Christopher Anderton, Kiran Bhadriraju, Anne L Plant, Brian G Bush, Robert Francis Cook, Frank W DelRio
Frictional properties of native and fibronectin (FN)-functionalized type I collagen (COL) thin films were studied via atomic force microscopy. The COL lateral contact stiffness was dependent only on the hydration state, indicating that shear deformat ...
5. Measurement Uncertainty in Cell Image Segmentation Data Analysis
NIST Interagency/Internal Report (NISTIR)
Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior,
and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is
fundamentally important to evaluate the performance ...
6. Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins
Antony K. Chen, Frank W DelRio, Alexander W Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L Plant
The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevan ...
7. ELUCIDATING THE SURFACE COMPOSITION OF MECHANICALLY MODULATED COLLAGEN FIBRIL FILMS WITH TOF-SIMS
Christopher R Anderton, Kiran Bhadriraju, Frank W DelRio, Anne L Plant
The extracellular matrix (ECM) environment plays a critical role in organism development and disease. Consequently, developing
complementary analytical techniques to study bioactive scaffolds can assist in understanding cell-ECM processes. We have ...
8. Review: Biological Imaging Software Tools
Anne L Plant, Kevin Eliceiri, Anne Carpenter
Few technologies are more widespread in modern biological laboratories than imaging by microscopy. Advancements in optical technologies and instrumentation over the past twenty years have led to massive improvements in the resolution, specificity, d ...
9. New Concepts for Building Vocabulary for Cell Image Ontologies
Anne L Plant, John T Elliott, Talapady N Bhat
Background: We present an approach to cell image databasing that is compatible with searching across a global federation of independent image databases. The variety of biological experiments and data that practitioners would like to access and share ...
10. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...