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Author: margaret kline

Displaying records 21 to 30 of 49 records.
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21. Testing Candidate DNA Quantitation Standards with Several Real-Time Quantitative PCR Methods
Published: 9/29/2005
Authors: Margaret C Kline, Peter M Vallone, Amy E. Decker, Janette W. Redman, David Lee Duewer, John M Butler
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908137

22. Chromosomal Duplications Along the Y-Chromosome and Their Potential Impact on Y-STR Interpretation
Published: 7/1/2005
Authors: John M Butler, Amy E. Decker, Margaret C Kline, Peter M Vallone
Abstract: Y-chromosome short tandem repeat (Y-STR) markers are being touted as potential tools for distinguishing low levels of male DNA in the presence of excess female DNA as is present in many sexual assault samples. Usually single copy Y-STR loci produce a ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830465

23. Results From the NIST 2004 DNA Quantitation Study
Published: 5/1/2005
Authors: Margaret C Kline, David Lee Duewer, Janette W. Redman, John M Butler
Abstract: For optimal DNA Short Tandem Repeat (STR) typing results, the DNA concentration ([DNA]) of the sample must be accurately determined prior to the polymerase chain reaction (PCR) amplification step in the typing process. In early 2004, the National In ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830467

24. Mitochondrial DNA Typing Screens With Control Region and Coding Region SNPs
Published: 3/1/2005
Authors: Margaret C Kline, Peter M Vallone, Janette W. Redman, David Lee Duewer, C D Calloway, John M Butler
Abstract: Mitochondrial DNA (mtDNA) analysis has found an important niche in forensic DNA typing. It is used with highly degraded samples or low-copy number materials such as might be found from shed hair or bones exposed to severe environmental conditions. ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830461

25. NIST Mixed Stain Study 3: Signal Intensity Balance in Commercial Short Tandem Repeat Multiplexes
Published: 12/1/2004
Authors: David Lee Duewer, Margaret C Kline, Janette W. Redman, John M Butler
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903148

26. High-Throughput Y-STR Typing of U.S. Populations With 27 Regions of the Y Chromosome Using Two Multiplex PCR Assays
Published: 1/28/2004
Authors: R Schoske, Peter M Vallone, Margaret C Kline, Janette W. Redman, John M Butler
Abstract: Two Y-chromosome short tandem repeat (STR) multiplex polymerase chain reaction (PCR) assays were used to generate haplotypes for 19 single copy and 3 multi-copy Y-STRs. A total of 27 PCR products were examined in each sample using the following loci ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830414

27. Biotech SRMs Designed for the Rapidly Evolving Forensic DNA and Human Identity Testing Communities
Published: 8/1/2003
Authors: Margaret C Kline, David Lee Duewer, John M Butler
Abstract: As advances in biotechnology have been applied to human identification, the National Institute of Standards and Technology (NIST) has provided Standard Reference Material (SRM ) to aid the forensic community to assure the quality of their measuremen ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830390

28. Allele Frequencies for 15 Autosomal STIR Loci on U.S. Caucasian, African American, and Hispanic Populations
Published: 7/1/2003
Authors: John M Butler, R Schoske, Peter M Vallone, Janette W. Redman, Margaret C Kline
Abstract: A total of 700 DNA samples from 3 U.S. Populations were examined at 15 different short tandem repeat (STR) markers commonly used is human identity testing. this manuscript simply describes the allele frequencies observed with our samples, which were ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830395

29. NIST Mixed Stain Study #3: DNA Quantitation Accuracy and its Influence on Short Tandem Repeat Multiplex Signal Intensity
Published: 5/15/2003
Authors: Margaret C Kline, David Lee Duewer, Janette W. Redman, John M Butler
Abstract: The Mixed Stain Study #3 (MSS3) interlaboratory challenge exercise evaluated the 2001 performance of STR multiplex DNA typing systems using a set of seven DNA extracts of designed concentration and composition. This initial report focuses on the lin ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830347

30. A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers
Published: 9/10/2002
Authors: John M Butler, R Schoske, Peter M Vallone, Margaret C Kline, A J Redd, M F Hammer
Abstract: A multiplex PCR assay capable of simultaneously amplifying 20 Y-chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the extend ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830288



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