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You searched on: Author: marcia holden

Displaying records 11 to 20 of 44 records.
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11. Structural analysis of ligand binding and catalysis in chorismate lyase
Published: 1/1/2006
Authors: N. Smith, A. Roitberg, E. Rivera, A. Howard, Marcia J Holden, M. Mayhew, S. Kaistha, David Travis Gallagher

12. An Inter-Platform Repeatability Study Investigating Real-Time Amplification of Plasmid DNA
Published: 5/25/2005
Authors: C Donald, F Qureshi, M J Burns, Marcia J Holden, J L. Blasic, A J Woolford
Abstract: Background: Currently, there are many different real-time amplification platforms. However, the results of DNA measurements from experiments performed on different platforms are not necessarily comparable and results may not be repeatable within inst ...

13. Spectroscopic Characterization of Fluorescein- and Tetramethylrhodamine-Labeled Oligonucleotides and Their Complexes With a DNA Template
Published: 10/1/2004
Authors: Lili Wang, Adolfas Kastytis Gaigalas, J L. Blasic, Marcia J Holden
Abstract: We measured absorption and emission spectra, fluorescence quantum yield, anisotropy, fluorescence resonance energy transfer (FRET), and melting temperature to characterize fluorescein- and tetramethyIrhodamine (TMR)-labeled oligonucleotides in soluti ...

14. Structure of Alanine Dehydrogenase from Archaeoglobus: Active Site Analysis and Relation to Bacterial Cyclodeaminases and Mammalian mu Crystallin
Published: 9/3/2004
Authors: David Travis Gallagher, H G Monbouquette, I Schroder, Hugh Robinson, Marcia J Holden, N Smith
Abstract: The hyperthermophilic archaeon Archaeoglobus fulgidus contains an L-Ala dehydrogenase (AlaDH, EC that is not homologous to known bacterial dehydrogenases and appears to represent a previously unrecognized archaeal group of NAD-dependent dehy ...

15. Structural Changes that Accompany the Temperature Dependence of the Formal Potential of Putidaredoxin
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker

16. Temperature-Induced Structural Changes in Putidaredoxin: A Circular Dichroism and UV-VIS Absorption Study
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Putidaredoxin (Pdx) is an 11,400 Da iron-sulfur protein that sequentially transfers two electrons to the cytochrome P450cam during the enzymatic cycle of the stereo specific camphor hydroxylation. We report two transitions in the Pdx UV-VIS absorpt ...

17. Temperature-induced structural changes in putidaredoxin: a circular dichroism and UV-VIS absorption study
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker

18. Structure of C73G putidaredoxin from Pseudomonas putida
Published: 5/1/2004
Authors: N Smith, M P. Mayhew, Marcia J Holden, H Kelly, H Robinson, A Heroux, V L. Vilker, David Travis Gallagher

19. Crystallization and Phasing of Alanine Dehydrogenase From Archaeoglobus Fulgidus
Published: 12/1/2003
Authors: N Smith, M P. Mayhew, Hugh Robinson, A Heroux, D Charlton, Marcia J Holden, David Travis Gallagher
Abstract: Alanine dehydrogenase (AlaDH) from the hyperthermophilic archaeon A. fudgidus is a dimer of 35 kdal chains. The gene (AF1665) is annotated as an ornithine cyclodeaminase based on homology with the ornithine deaminase/ mu crystallin enzyme family. I ...

20. Evaluation of Extraction Methodologies for Corn Kernal (Zea Mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA
Published: 4/23/2003
Authors: Marcia J Holden, J L. Blasic, L Bussjaeger, C Kao, Luke A. Shokere, D C Kendall, L Freese, G. Ronald Jenkins
Abstract: Sensitivity and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were ev ...

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