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Author: marcia holden

Displaying records 11 to 20 of 43 records.
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11. An Inter-Platform Repeatability Study Investigating Real-Time Amplification of Plasmid DNA
Published: 5/25/2005
Authors: C Donald, F Qureshi, M J Burns, Marcia J Holden, J L. Blasic, A J Woolford
Abstract: Background: Currently, there are many different real-time amplification platforms. However, the results of DNA measurements from experiments performed on different platforms are not necessarily comparable and results may not be repeatable within inst ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830446

12. Spectroscopic Characterization of Fluorescein- and Tetramethylrhodamine-Labeled Oligonucleotides and Their Complexes With a DNA Template
Published: 10/1/2004
Authors: Lili Wang, Adolfas Kastytis Gaigalas, J L. Blasic, Marcia J Holden
Abstract: We measured absorption and emission spectra, fluorescence quantum yield, anisotropy, fluorescence resonance energy transfer (FRET), and melting temperature to characterize fluorescein- and tetramethyIrhodamine (TMR)-labeled oligonucleotides in soluti ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830426

13. Structure of Alanine Dehydrogenase from Archaeoglobus: Active Site Analysis and Relation to Bacterial Cyclodeaminases and Mammalian mu Crystallin
Published: 9/3/2004
Authors: David Travis Gallagher, H G Monbouquette, I Schroder, Hugh Robinson, Marcia J Holden, N Smith
Abstract: The hyperthermophilic archaeon Archaeoglobus fulgidus contains an L-Ala dehydrogenase (AlaDH, EC 1.4.1.1) that is not homologous to known bacterial dehydrogenases and appears to represent a previously unrecognized archaeal group of NAD-dependent dehy ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830482

14. Structural Changes that Accompany the Temperature Dependence of the Formal Potential of Putidaredoxin
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903189

15. Temperature-Induced Structural Changes in Putidaredoxin: A Circular Dichroism and UV-VIS Absorption Study
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Putidaredoxin (Pdx) is an 11,400 Da iron-sulfur protein that sequentially transfers two electrons to the cytochrome P450cam during the enzymatic cycle of the stereo specific camphor hydroxylation. We report two transitions in the Pdx UV-VIS absorpt ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830425

16. Temperature-induced structural changes in putidaredoxin: a circular dichroism and UV-VIS absorption study
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903187

17. Structure of C73G putidaredoxin from Pseudomonas putida
Published: 5/1/2004
Authors: N Smith, M P. Mayhew, Marcia J Holden, H Kelly, H Robinson, A Heroux, V L. Vilker, David Travis Gallagher
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906585

18. Crystallization and Phasing of Alanine Dehydrogenase From Archaeoglobus Fulgidus
Published: 12/1/2003
Authors: N Smith, M P. Mayhew, Hugh Robinson, A Heroux, D Charlton, Marcia J Holden, David Travis Gallagher
Abstract: Alanine dehydrogenase (AlaDH) from the hyperthermophilic archaeon A. fudgidus is a dimer of 35 kdal chains. The gene (AF1665) is annotated as an ornithine cyclodeaminase based on homology with the ornithine deaminase/ mu crystallin enzyme family. I ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830329

19. Evaluation of Extraction Methodologies for Corn Kernal (Zea Mays) DNA for Detection of Trace Amounts of Biotechnology-Derived DNA
Published: 4/23/2003
Authors: Marcia J Holden, J L. Blasic, L Bussjaeger, C Kao, Luke A. Shokere, D C Kendall, L Freese, G. Ronald Jenkins
Abstract: Sensitivity and accurate testing for trace amounts of biotechnology-derived DNA from plant material requires pure, high-quality genomic DNA as template for subsequent amplification using the polymerase chain reaction (PCR). Six methodologies were ev ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830357

20. Fluorescence Resonance Energy Transfer Between Donor-Acceptor Pair on Two Oligonucleotides Hybridized Adjacently to a DNA Template
Published: 1/1/2003
Authors: Lili Wang, Adolfas Kastytis Gaigalas, J L. Blasic, Marcia J Holden, David Travis Gallagher, R Pires
Abstract: We have used fluorescein as the energy donor and rhodamine as the acceptor to measure the efficiency of fluorescence resonance energy transfer (FRET) in a set of hybridized DNA constructs. The two fluorophores are covalently attached via linkers to t ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830377



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