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1. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

2. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901347

3. Automated Ranking of Stem Cell Colonies by Translating Biological Rules to Computational Models
Published: 9/20/2014
Authors: Adele P Peskin, Steven P Lund, YaShian Li-Baboud, Michael W Halter, Anne L Plant, Peter Bajcsy
Abstract: This paper addresses the problem of automating an image ranking process for stem cell colonies. We automate the manual process in a novel way: instead of fitting off-the-shelf image features and colony ranks to prediction models, we define a new feat ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915931

4. Boltzmann's H-Function and Diffusion Processes
Published: 5/15/2013
Authors: Steven P Lund, Joseph Bunton Hubbard, Michael W Halter
Abstract: There exists a generalization of Boltzmann's $H$-function that allows for non-uniformly populated stationary states which may exist far from thermodynamic equilibrium. Here we describe a method for obtaining a generalized or collective diffusion coef ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913439

5. Cell volume distributions reveal cell growth rates and division times
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832284

6. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Published: 6/14/2011
Authors: Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Abstract: Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905998

7. Dimensional Metrology of Lab on a Chip Internal Structures: a Comparison of Optical Coherence Tomography with Coherence Fluorescence Microscopy
Published: 4/8/2015
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: The characterization of internal structures in a polymeric device, specifically of a final product, will require a different set of metrology techniques than those traditionally use in the characterization of microelectronic devices. OCT is relative ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912533

8. Evaluation of Segmentation Algorithms on Cell Populations Using CDF Curves
Published: 2/24/2012
Authors: Robert Charles Hagwood, Javier Bernal, Michael W Halter, John T Elliott
Abstract: Cell segmentation is a critical step in the analysis pipeline for most imaging cytometry experiments and the segmentation algorithm can effect the quantitative data derived from image analysis. Methods to evaluate segmentation algorithms are importan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907743

9. High Resolution Surface Plasmon Resonance Imaging for Single Cells
Published: 12/1/2014
Authors: Alexander W Peterson, Michael W Halter, Alessandro Tona, Anne L Plant
Abstract: Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However, c ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914643

10. Measurement Uncertainty in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7954
Published: 8/13/2013
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Abstract: Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914171



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