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Author: michael halter
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1. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

2. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901347

3. Boltzmann's H-Function and Diffusion Processes
Published: 5/15/2013
Authors: Steven P Lund, Joseph Bunton Hubbard, Michael W Halter
Abstract: There exists a generalization of Boltzmann's $H$-function that allows for non-uniformly populated stationary states which may exist far from thermodynamic equilibrium. Here we describe a method for obtaining a generalized or collective diffusion coef ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913439

4. Cell volume distributions reveal cell growth rates and division times
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832284

5. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Published: 6/14/2011
Authors: Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Abstract: Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905998

6. Measurement Uncertainty in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7954
Published: 8/13/2013
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Abstract: Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914171

7. Metrology for Lab-on-a-Chip Final-Product Devices
Published: 5/12/2013
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: New metrology tools to measure the critical parameters of internal structures in lab on a chip devices are greatly needed in order to develop standard tests for this technology. Here we present a method that combines a custom made optical coherence ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913496

8. Optical Coherence Tomography for Dimensional Metrology of Lab-on-a-chip devices
Published: 10/27/2013
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: Here we present a new metrology tool for the determination of the dimensions of channels from final product Lab-on-a-chip devices. Using Optical Coherence Tomography (OCT) areas of up to 12 mm x 12 mm can be imaged in less than 1 second, without the ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914314

9. Overlap-Based Cell Tracker
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7663
Published: 2/2/2010
Authors: Joe Chalfoun, Antonio Cardone, Alden A Dima, Michael W Halter, Daniel P Allen
Abstract: In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an introduction to the general principle of an overlap cell tracking software developed by NIST. Th ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904008

10. Package "cellVolumeDist"
Published: 4/13/2010
Authors: Katharine M Mullen, Michael W Halter
Abstract: This package implements a methodology for using cell volume distributions to estimate cell growth rates and division times that is described in the paper entitled "Cell Volume Distributions Reveal Cell Growth Rates and Division Times", by M ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901522



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