NIST logo
*

Micronutrients Measurement Quality Assurance Program

Summary:


The Micronutrients Measurement Quality Assurance Program, coordinated by the Chemical Sciences Division, supports measurement technology for selected fat- and water-soluble vitamins and carotenoids in human serum. This program was initiated in 1984 by the National Cancer Institute Division of Cancer Prevention and Control to ensure the long-term reliability of the measurements made while studying the possible cancer chemoprevention roles of these compounds. From an initial core of 10 NCI-grantee laboratories, the program currently involves 34 fee-paying laboratories. This program provides participants with measurement comparability assessment through use of interlaboratory comparison studies, Standard Reference Materials and control materials, and methods development and validation.

Description:


NIST Micronutrients Measurement Quality Assurance (QA) Program supports the long-term (months to years) reliability of selected fat- or water-soluble vitamin measurements in human serum and plasma. Results from the comparison studies help participants to make accurate clinical and health-care decisions as well as to maintain and improve their measurement comparability. Laboratories use the program to demonstrate their compliance with federally regulated requirements [Clinical Laboratory Improvement Act (CLIA)], which extend to all laboratories performing diagnostic testing.

Additional Technical Details:


Serum-based samples with assigned values for target analytes (e.g., retinol, tocopherols, beta--carotene, and ascorbic acid) and performance-evaluation standards are distributed by NIST to participants for analysis. NIST staff provide participants with technical feedback concerning their measurement performance and suggestions for methods development and refinement. A certificate of participation in the QA program is issued at the end of each calendar year.

Major Accomplishments:

The Micronutrients Measurement QA Program at NIST has administered over 90 interlaboratory comparison exercises for the measurement of selected fat- and water-soluble vitamins in human serum and plasma. The comparability of laboratory measurements for target analytes has improved substantially over time. This has been achieved through the development and promulgation of robust measurement technologies, identification and production of suitable reference materials, isolation and identification of measurement system biases, and support and encouragement of long-term within-laboratory measurement quality control efforts. The average estimated coefficient of variation for retinol and α-tocopherol has been approximately 5% for the past seven years and about 10% for β-carotene for that same period of time.

For program information, including how to join, click here.

micronuts qap

Start Date:

October 1, 1984

End Date:

Ongoing

Lead Organizational Unit:

mml

Customers/Contributors/Collaborators:

ARUP Laboratories, Salt Lake City, UT, USA
Bio-Reference Laboratories, Elmwood Park, NJ, USA
Cancer Geriatrics Center, University of Michigan, Ann Arbor, MI, USA
Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, HI, USA
Centers for Disease Control and Prevention, Atlanta, GA, USA
Biochemical Genetics Laboratory, Duke University, Research Triangle Park, NC, USA
Biochemical Genetics Laboratory, Mayo Clinic, Rochester, MN, USA
Biochemical Genetics Laboratory, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
Children’s Hospital and Regional Medical Center, Seattle, WA, USA
Children’s Hospital National Medical Center, Washington, DC, USA
Department of Human Nutrition, University of Illinois at Chicago, Chicago, IL, USA
Department of Human Nutrition, University of Stellenbosch, Tygerberg Campus, Tygerberg, South Africa
Department of Laboratory Medicine and Pathology, University of Alberta Hospital, Alberta, Canada
Department of Nutrition, Harvard School of Public Health, Boston, MA, USA
Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA
ESA Laboratories, Inc., Chelmsford, MA, USA
Fred Hutchinson Cancer Research Center, Seattle, WA, USA
Global Central Laboratory, Highland Heights, KY, USA
Harborview Medical Center, University of Washington, Seattle, WA, USA
Human Nutrition Unit, National Institute for Food and Nutritional Research, Rome, Italy
International Centre for Diarrhoeal Diseases Research, Dhaka, Bangladesh
Kronos Science Laboratory, Phoenix, AZ, USA
Laboratoire de Biochimie, Hôpital Purpan, Toulouse, France
Life Sciences Group, Wyle Laboratories, Inc., Houston, TX, USA
MetaMetrix Medical Laboratory, Duluth, GA, USA
MRC Laboratory for Human Nutrition Research, Cambridge, England
Neonatal Nutrition Research Laboratory, University of Louisville, Louisville, KY, USA
Nutrition Research Laboratory, University of California at San Diego, La Jolla, CA, USA     
Pediatric CTRC CORE Laboratory, University of Colorado Health Sciences Center, Denver, CO, USA
Quest Diagnostics, Inc., Chantilly, VA, USA
R&D Analytical Research Center, DSM Nutritional Products, Ltd., Kaiseraugst, Switzerland
Rowett Research Institute, Aberdeen, Scotland
Servicio de Endocrinologia y Nutricion, Hospital Universitario Puerta de Hierro, Madrid, Spain

Associated Products:

If you or your institution subscribe to the journal cited below, clicking on the link should take you to a page from which you can access the reference.

Brown Thomas, J. and Sharpless, K. E., eds., Methods for Analysis of Cancer Chemopreventive Agents in Human Serum, NIST Special Publication 874, U. S. Gov't Printing Office, Washington, DC, 128 pages, (1995).

Sharpless, K. E.; Duewer, D. L. Population Distributions and Intralaboratory Variance for Fat-Soluble Vitamin-Related Compounds in Human Serum, Anal. Chem., 67: 4416-4422 (1995).

Brown Thomas, J.; Kline, M. C.; Schiller, S. B.; Ellerbe, P. M.; Sniegoski, L. T.; Duewer, D. L.; Sharpless, K. E. Certification of Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum: Standard Reference Material 968b, Fresenius' J. Anal. Chem., 356:1-9 (1996). 

Margolis, S. A.; Duewer, D. L. The Measurement of Ascorbic Acid in Human Plasma and Serum: Intralaboratory Repeatability, Interlaboratory Repeatability, Interlaboratory Reproducibility, and Stability in Lyophilized Plasma and Metaphosphoric Acid Preserved Plasma and Serum, Clin. Chem., 42: 1257-1262 (1996).

Duewer, D.L., Brown Thomas, J. Kline, M.C., MacCrehan, W. A., Schaffer, R., Sharpless, K.E., May, W.E., “NIST/NCI Micronutrients Measurement Quality Assurance Program: Measurement Reproducibility, Repeatability, Stability, and Relative Accuracy for Fat-Soluble Vitamin-Related Compounds in Human Sera,” Anal. Chem. 69: 1406-1413 (1997).

Margolis, S.A. and Schapira, R.M. Liquid Chromatographic Measurement of L-Ascorbic Acid and D-Ascorbic Acid in Biological Samples, J. Chromatogr. B, 690: 25-33 (1997).

Sharpless, K.S., Brown Thomas, J., Turley, C.P., and Brewster, M.A., “Vitamin A,” In: LaboratoryMedicine: A Scientific and Managerial Infobase, Version 2.6, A.J.Pesce and L.A. Kaplan, (eds.) Pesce Kaplan Publisher, Cincinnati, OH, CD-Rom (1997).

Sharpless, K.S., Brown Thomas, J., Turley, C.P., and Brewster, M.A., “Vitamin E,” In: Laboratory Medicine: A Scientific and Managerial Infobase, Version 2.6, A.J.Pesce and L.A. Kaplan, (eds.) Pesce Kaplan Publisher, Cincinnati, OH, CD-Rom (1997).

Brown Thomas, J.; Kline, M. C.; Duewer, D. L.; Sharpless, K. E, The Stability of Retinol, α-Tocopherol, Trans-Lycopene, and Trans- β-Carotene in Liquid-frozen and Lyophilized Serum in Human Serum, Clin. Chim. Acta, 276:75-87 (1998).

Duewer, D. L.; Kline, M. C.; Sharpless, K. E.; Brown Thomas, J. M.; Gary, K. T.; Sowell, A. L. Micronutrients Measurement Quality Assurance Program: Helping Participants Use Inter-laboratory Comparison Exercise Results to Improve their Long-term Measurement Performance. Anal. Chem., 71: 1870 -1878 (1999).

Duewer. D.L., Kline, M.C., Sharpless, K.E., Brown Thomas, J., Stacewicz-Sapuntzakis, M., And Sowell, A.L., “NIST Micronutrients Measurement Quality Assurance Program: Characterizing Individual Participant Measurement Performance Over Time,” Anal. Chem., 72: 3611- 3619 (2000).

Duewer. D.L., Kline, M.C., Sharpless, K.E., and Brown Thomas, J., “NIST Micronutrients Measurement Quality Assurance Program: Characterizing the Measurement Community’s Performance Over Time,” Anal. Chem., 72: 4163 - 4170 (2000).

Brown Thomas, J., Kline, M.C., Gill, L.M., Yen, J.H., Duewer, D.L., Sniegoski, L.T., and Sharpless, K.E., “Preparation and Value Assignment of Constituents in Standard Reference Material 968c: Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum,” Clin. Chim. Acta, 305:141 -155 (2001).

Contact

Jeanice B. Thomas
Phone 301-975-3120
jbthomas@nist.gov

David L. Duewer
Phone 301-975-3945
david.duewer@nist.gov 

Lane C. Sander
Phone 301-975-3117
lane.sander@nist.gov