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Micronutrients Measurement Quality Assurance Program

Summary:

The Micronutrients Measurement Quality Assurance Program, coordinated by the Chemical Sciences Division, supports measurement technology for selected fat- and water-soluble vitamins and carotenoids in human serum. This program was initiated in 1984 by the National Cancer Institute Division of Cancer Prevention and Control to ensure the long-term reliability of the measurements made while studying the possible cancer chemoprevention roles of these compounds. From an initial core of 10 NCI-grantee laboratories, the program currently involves 34 fee-paying laboratories. This program provides participants with measurement comparability assessment through use of interlaboratory comparison studies, Standard Reference Materials and control materials, and methods development and validation.

Description:

NIST Micronutrients Measurement Quality Assurance (QA) Program supports the long-term (months to years) reliability of selected fat- or water-soluble vitamin measurements in human serum and plasma. Results from the comparison studies help participants to make accurate clinical and health-care decisions as well as to maintain and improve their measurement comparability. Laboratories use the program to demonstrate their compliance with federally regulated requirements [Clinical Laboratory Improvement Act (CLIA)], which extend to all laboratories performing diagnostic testing.

Additional Technical Details:

Serum-based samples with assigned values for target analytes (e.g., retinol, tocopherols, and beta--carotene and performance-evaluation standards are distributed by NIST to participants for analysis. NIST staff provide participants with technical feedback concerning their measurement performance and suggestions for methods development and refinement. A certificate of participation in the QA program is issued at the end of each calendar year.

Major Accomplishments:

The Micronutrients Measurement QA Program at NIST has administered over 90 interlaboratory comparison exercises for the measurement of selected fat- and water-soluble vitamins in human serum and plasma. The comparability of laboratory measurements for target analytes has improved substantially over time. This has been achieved through the development and promulgation of robust measurement technologies, identification and production of suitable reference materials, isolation and identification of measurement system biases, and support and encouragement of long-term within-laboratory measurement quality control efforts. The average estimated coefficient of variation for retinol and α-tocopherol has been approximately 5% for the past seven years and about 10% for β-carotene for that same period of time.

For program information, including how to join, click here.

micronuts qap

Start Date:

October 1, 1984

End Date:

Ongoing

Lead Organizational Unit:

mml

Customers/Contributors/Collaborators:

  • ARUP Laboratories, Salt Lake City, UT, USA
  • Bio-Reference Laboratories, Elmwood Park, NJ, USA
  • Cancer Geriatrics Center, University of Michigan, Ann Arbor, MI, USA
  • Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, HI, USA
  • Centers for Disease Control and Prevention, Atlanta, GA, USA
    Biochemical Genetics Laboratory, Duke University, Research Triangle Park, NC, USA
  • Biochemical Genetics Laboratory, Mayo Clinic, Rochester, MN, USA
  • Biochemical Genetics Laboratory, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
  • Children’s Hospital and Regional Medical Center, Seattle, WA, USA
  • Children’s Hospital National Medical Center, Washington, DC, USA
  • Department of Human Nutrition, University of Illinois at Chicago, Chicago, IL, USA
  • Department of Human Nutrition, University of Stellenbosch, Tygerberg Campus, Tygerberg, South Africa
  • Department of Laboratory Medicine and Pathology, University of Alberta Hospital, Alberta, Canada
  • Department of Nutrition, Harvard School of Public Health, Boston, MA, USA
  • Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA
  • ESA Laboratories, Inc., Chelmsford, MA, USA
  • Fred Hutchinson Cancer Research Center, Seattle, WA, USA
  • Global Central Laboratory, Highland Heights, KY, USA
  • Harborview Medical Center, University of Washington, Seattle, WA, USA
  • Human Nutrition Unit, National Institute for Food and Nutritional Research, Rome, Italy
  • International Centre for Diarrhoeal Diseases Research, Dhaka, Bangladesh
  • Kronos Science Laboratory, Phoenix, AZ, USA
  • Laboratoire de Biochimie, Hôpital Purpan, Toulouse, France
  • Life Sciences Group, Wyle Laboratories, Inc., Houston, TX, USA
  • MetaMetrix Medical Laboratory, Duluth, GA, USA
  • MRC Laboratory for Human Nutrition Research, Cambridge, England
  • Neonatal Nutrition Research Laboratory, University of Louisville, Louisville, KY, USA
  • Nutrition Research Laboratory, University of California at San Diego, La Jolla, CA, USA
  • Pediatric CTRC CORE Laboratory, University of Colorado Health Sciences Center, Denver, CO, USA
  • Quest Diagnostics, Inc., Chantilly, VA, USA
  • R&D Analytical Research Center, DSM Nutritional Products, Ltd., Kaiseraugst, Switzerland
  • Rowett Research Institute, Aberdeen, Scotland
  • Servicio de Endocrinologia y Nutricion, Hospital Universitario Puerta de Hierro, Madrid, Spain

Associated Products:

If you or your institution subscribe to the journal cited below, clicking on the link should take you to a page from which you can access the reference.

Brown Thomas, J., Sharpless, K.E., eds., Methods for Analysis of Cancer Chemopreventive Agents in Human Serum, NIST Special Publication 874, U. S. Gov't Printing Office, Washington, DC, 128 pages, (1995).

Sharpless, K.E., Duewer, D.L., Population Distributions and Intralaboratory Variance for Fat-Soluble Vitamin-Related Compounds in Human Serum, Anal. Chem., 67: 4416-4422 (1995).

Brown Thomas, J., Kline, M.C., Schiller, S.B., Ellerbe, P.M., Sniegoski, L.T., Duewer, D.L., Sharpless, K.E., Certification of Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum: Standard Reference Material 968b, Fresenius' J. Anal. Chem., 356:1-9 (1996). 

Margolis, S.A.; Duewer, D.L., Measurement of Ascorbic Acid in Human Plasma and Serum: Intralaboratory Repeatability, Interlaboratory Repeatability, Interlaboratory Reproducibility, and Stability in Lyophilized Plasma and Metaphosphoric Acid Preserved Plasma and Serum, Clin. Chem., 42: 1257-1262 (1996).

Duewer, D.L., Brown Thomas, J., Kline, M.C., MacCrehan, W.A., Schaffer, R., Sharpless, K.E., May, W.E., NIST/NCI Micronutrients Measurement Quality Assurance Program: Measurement Reproducibility, Repeatability, Stability, and Relative Accuracy for Fat-Soluble Vitamin-Related Compounds in Human Sera, Anal. Chem., 69: 1406-1413 (1997).

Margolis, S.A., Schapira, R.M., Liquid Chromatographic Measurement of L-Ascorbic Acid and D-Ascorbic Acid in Biological Samples, J. Chromatogr. B, 690: 25-33 (1997).

Sharpless, K.S., Brown Thomas, J., Turley, C.P., Brewster, M.A., “Vitamin A,” In: LaboratoryMedicine: A Scientific and Managerial Infobase, Version 2.6, A.J. Pesce and L.A. Kaplan, (eds.) Pesce Kaplan Publisher, Cincinnati, OH, CD-Rom (1997).

Sharpless, K.S., Brown Thomas, J., Turley, C.P., Brewster, M.A., “Vitamin E,” In: Laboratory Medicine: A Scientific and Managerial Infobase, Version 2.6, A.J. Pesce and L.A. Kaplan, (eds.) Pesce Kaplan Publisher, Cincinnati, OH, CD-Rom (1997).

Brown Thomas, J., Kline, M.C., Duewer, D.L., Sharpless, K.E, The Stability of Retinol, α-Tocopherol, Trans-Lycopene, and Trans- β-Carotene in Liquid-frozen and Lyophilized Serum in Human Serum, Clin. Chim. Acta, 276:75-87 (1998).

Duewer, D. L., Kline, M.C., Sharpless, K.E., Brown Thomas, J.M., Gary, K.T., Sowell, A.L., Micronutrients Measurement Quality Assurance Program: Helping Participants Use Inter-laboratory Comparison Exercise Results to Improve their Long-term Measurement Performance, Anal. Chem., 71: 1870 -1878 (1999).

Duewer. D.L., Kline, M.C., Sharpless, K.E., Brown Thomas, J., Stacewicz-Sapuntzakis, M., Sowell, A.L., NIST Micronutrients Measurement Quality Assurance Program: Characterizing Individual Participant Measurement Performance Over Time, Anal. Chem., 72: 3611- 3619 (2000).

Duewer. D.L., Kline, M.C., Sharpless, K.E., Brown Thomas, J., NIST Micronutrients Measurement Quality Assurance Program: Characterizing the Measurement Community’s Performance Over Time, Anal. Chem., 72: 4163-4170 (2000).

Brown Thomas, J., Kline, M.C., Gill, L.M., Yen, J.H., Duewer, D.L., Sniegoski, L.T., Sharpless, K.E., Preparation and Value Assignment of Constituents in Standard Reference Material 968c: Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum, Clin. Chim. Acta, 305:141 -155 (2001).

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