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Bioanalytical Science Group

Welcome

We provide the measurement science, standards, technology, and data required to support the nation's needs in determining the composition, structure, quantity, and function of proteins, peptides, post-translational modifications such as glycosylation and phosphorylation, and related biomolecules such as metabolites.  In partnership with U.S. industry, government agencies, and scientific institutions, the group performs fundamental and applied research on the characterization and quantitation of proteins, peptides, and their mixtures using techniques such as mass spectrometry, liquid chromatography, electrophoresis, and immunoassays.  The Group provides measurement science, reference materials, reference data, and technologies to support accuracy and comparability of measurements needed to advance biotechnology, biomedical and bioscience research, biomanufacturing, and health care.  Specific areas of interest include measurements and standards for clinical diagnostics and biotherapeutics.

Programs/Projects

Development of Proteomics-Based Methods for Protein Quantification— NIST is developing methods for the quantification of clinically-relevant protein biomarkers by mass spectrometry. Some methods are intended to establish reference measurement procedures for the …

Improving Measurement Quality in Clinical Proteomics— Through collaboration with academic and government institutes, NIST is critically evaluating and attempting to improve measurement quality in clinical mass spectrometric proteomic investigations. …

Development of Reference Methods and Reference Materials for the Determination of Cardiac Troponin I in Human Serum— Cardiac troponin I (cTnI) is considered the 'gold standard' for the diagnosis of myocardial infarction and the assessment of cardiac damage. Yet this clinical measurement is plagued with …

Development of a Reference Measurement Procedure for C-Reactive Protein in Human Serum— C-reactive protein (CRP) levels in serum or plasma have been used as an indicator of inflammation and infection. Recent evidence also suggests that CRP levels may have value in identifying …

Development of an Amino Acid (in Solution) Standard Reference Material— Amino acids are the sequential components of proteins and many are essential to our physiological well-being. Their quantification by the scientific community varies in technique and application. …

Chromatographic Separation of Unfolded Proteins for Protein Quantification— NIST is developing methods for the quantification of protein biomarkers by mass spectrometry (MS). Stable isotope labeling of proteins/peptides followed by MS analysis allows relative …

Development of Reference Methods and Reference Materials for Proteins — A number of proteins show promise as potential markers of health or disease, and commercial assays are being developed for these proteins. For example, measurement of the protein cardiac troponin …

 
 
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Employment/Research Opportunities
Contact

Karen Phinney, Group Leader

Phone: 301-975-4457
Fax: 301-977-0685
Email: karen.phinney@nist.gov