The ability to acquire large amounts of high quality data very rapidly is increasingly important for progress in the biological sciences. Capillary electrophoresis (CE) has a number of advantages that make it well suited to provide the kind of quantitative data that is necessary. However, high throughput CE instruments are complex and expensive, limiting their widespread use. This project is directed at the development of a new approach to multiplexed electrophoresis which is much simpler and less expensive. In the new approach, the bundle of long separation capillaries is replaced by an array of very short electrophoresis channels, and the multi-point optical detection system of the conventional CE approach is replaced by an array of electrical resistors. The new electrophoresis device can be made to fit into the format of a microtiter plate, making it compatible with existing robotic systems for high throughput measurements.
Additional Technical Details:
NIST has developed a new approach for multiplexed electrophoretic analysis. This “detectorless” approach is an implementation of the gradient elution moving boundary electrophoresis (GEMBE) technique, previously developed at NIST. With GEMBE, a combination of an electric field and controlled, variable buffer counterflow is used to achieve high resolution separations of multiple analyte species. Typically, the polarity of the applied voltage is set so that the charged analytes are driven by electrophoresis from the sample toward the separation channel. At the beginning of a separation, the counterflow is high so that none of the analytes of interest will enter the channel. Over the course of the separation, the counterflow is gradually reduced so that each analyte, in turn, will enter the channel as a moving boundary or step. For “detectorless” analysis, very short separation channels (< 3 mm) are used, so that only one step at a time is present in each channel. Consequently, measurement of the current through each electrophoresis channel can be used in place of a detector signal, with essentially no detector hardware. The time derivative of the current is then equivalent to a conventional electropherogram. Because of the simplicity of the hardware used, the “detectorless” electrophoresis approach is simple and inexpensive to multiplex; making it ideally suited to high throughput measurements.
Fraction of substrate converted to product vs. inhibitor concentration for various time points.
Start Date:December 3, 2007
Lead Organizational Unit:mml