NIST logo

Publication Citation: High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

NIST Authors in Bold

Author(s): Charles H. Camp; Young J. Lee; John Heddleston; Christopher M. Hartshorn; Angela R. Hight Walker; Jeremy N. Rich; Justin D. Lathia; Marcus T. Cicerone;
Title: High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
Published: July 20, 2014
Abstract: We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3000 cm−1 and generates an especially strong response in the typically weak Raman ,fingerprintŠ region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of < 13 cm−1. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter.
Citation: Nature Photonics
Volume: 8
Pages: pp. 627 - 634
Keywords: broadband coherent anti-Stokes Raman scattering; chemical imaging; coherent anti-Stokes Raman scattering; hyperspectral imaging; label-free
Research Areas: Optics, Molecular Pathology, Quantitative Molecular Imaging, Physical Measurements, Academic, Raman, Nonlinear Optical Spectroscopy, Imaging, Spectroscopy
DOI: http://dx.doi.org/10.1038/nphoton.2014.145  (Note: May link to a non-U.S. Government webpage)
PDF version: PDF Document Click here to retrieve PDF version of paper (3MB)