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|Author(s):||Charles H. Camp; Young J. Lee; John M. Heddleston; Christopher M. Hartshorn; Angela R. Hight Walker; Jeremy N. Rich; Justin D. Lathia; Marcus T. Cicerone;|
|Title:||High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues|
|Published:||July 20, 2014|
|Abstract:||We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3000 cm−1 and generates an especially strong response in the typically weak Raman ,fingerprintŠ region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of < 13 cm−1. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter.|
|Pages:||pp. 627 - 634|
|Keywords:||broadband coherent anti-Stokes Raman scattering, chemical imaging, coherent anti-Stokes Raman scattering, hyperspectral imaging, label-free|
|Research Areas:||Optics, Molecular Pathology, Quantitative Molecular Imaging, Physical Measurements, Academic, Raman, Nonlinear Optical Spectroscopy, Imaging, Spectroscopy|
|DOI:||http://dx.doi.org/10.1038/nphoton.2014.145 (Note: May link to a non-U.S. Government webpage)|
|PDF version:||Click here to retrieve PDF version of paper (3MB)|