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Publication Citation: Standard Practice for Determining the Relative Spectral Correction Factors for the Emission Signal of Fluorescence Spectrometers

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Author(s): Paul C. DeRose;
Title: Standard Practice for Determining the Relative Spectral Correction Factors for the Emission Signal of Fluorescence Spectrometers
Published: August 15, 2013
Abstract: This standard practice is intended to aid in the calibration and qualification of fluorescence spectrometers as part of complying with regulatory and other QA/QC requirements. It specifies the materials and procedures used in three different methods for the correction of the relative responsivity of the instrument's detection system as a function of wavelength, also known as relative spectral correction. ASTM Luminescence subcommittee E13.01.01 intends to use this document as a template for a similar ASTM guide.
Citation: NIST Interagency/Internal Report (NISTIR) - 7915
Keywords: biosystems and health; calibration; fluorescence; instrument qualification; method validation; reference materials; spectral correction
Research Areas: Standard Reference Materials, Bioscience & Health
PDF version: PDF Document Click here to retrieve PDF version of paper (187KB)