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Publication Citation: Facile Fabrication of "Dual Click" One- and Two-Dimensional Orthogonal Peptide Concentration Gradients

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Author(s): Yanrui Ma; Jukuan Zheng; Emily F. Amond; Christopher M. Stafford; Matthew L. Becker;
Title: Facile Fabrication of "Dual Click" One- and Two-Dimensional Orthogonal Peptide Concentration Gradients
Published: January 21, 2013
Abstract: Peptides, proteins and extracellular matrix act in concert to influence cellular function at the biotic-synthetic interface. However identifying the individual and synergistic contributions of the various combinations and concentration regimes is extremely difficult. The confined channel deposition method we describe affords highly tunable orthogonal reactive concentration gradients that greatly expand the dynamic range, spatial control, and chemical versatility of the reactive silanes that can be controllably deposited. Using metal-free ,dual clickŠ immobilization chemistries, peptides with a variety of functionality can be immobilized efficiently and reproducibly enabling optimal concentration profiling and the assessment of synergistic interactions.
Citation: Biomacromolecules
Volume: 14
Issue: 3
Pages: pp. 665 - 671
Keywords: gradients; monolayers; click chemistry; self-assembled monolayers; surface modification; functionalization
Research Areas: Biocompatability, Combinatorial Methods, Biomaterials, Surfaces
DOI: http://dx.doi.org/10.1021/bm301731h  (Note: May link to a non-U.S. Government webpage)