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|Author(s):||Yanrui Ma; Jukuan Zheng; Emily F. Amond; Christopher M. Stafford; Matthew L. Becker;|
|Title:||Facile Fabrication of "Dual Click" One- and Two-Dimensional Orthogonal Peptide Concentration Gradients|
|Published:||January 21, 2013|
|Abstract:||Peptides, proteins and extracellular matrix act in concert to influence cellular function at the biotic-synthetic interface. However identifying the individual and synergistic contributions of the various combinations and concentration regimes is extremely difficult. The confined channel deposition method we describe affords highly tunable orthogonal reactive concentration gradients that greatly expand the dynamic range, spatial control, and chemical versatility of the reactive silanes that can be controllably deposited. Using metal-free ,dual clickŠ immobilization chemistries, peptides with a variety of functionality can be immobilized efficiently and reproducibly enabling optimal concentration profiling and the assessment of synergistic interactions.|
|Pages:||pp. 665 - 671|
|Keywords:||gradients, monolayers, click chemistry, self-assembled monolayers, surface modification, functionalization|
|Research Areas:||Biocompatability, Combinatorial Methods, Biomaterials, Surfaces|
|DOI:||http://dx.doi.org/10.1021/bm301731h (Note: May link to a non-U.S. Government webpage)|