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Publication Citation: Determination of fortified and endogenous folates in food-based Standard Reference Materials by liquid chromatography-tandem mass spectrometry

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Author(s): Johanna Camara; Mark S. Lowenthal; Karen W. Phinney;
Title: Determination of fortified and endogenous folates in food-based Standard Reference Materials by liquid chromatography-tandem mass spectrometry
Published: January 26, 2013
Abstract: The National Institute of Standards and Technology (NIST) is developing a wide variety of Standard Reference Materials (SRMs) to support measurements of vitamins and other nutrients in foods. Previously, NIST has provided SRMs with values assigned for the folate vitamer, folic acid (pteroylglutamic acid), which is fortified in several foods due to its role in prevention of neural tube defects. In order to expand the number of food-based SRMs with values assigned for folic acid, as well as additional endogenous folates, NIST has developed methods that include trienzyme digestion and isotope-dilution liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Sample preparation was optimized for each individual food type, but all samples were analyzed under the same LC-MS/MS conditions. The application of these methods resulted in folic acid values for SRM 1849a Infant/Adult Nutritional Formula and SRM 3233 Fortified Breakfast Cereal of 2.33 ± 0.06 µg/g and 16.0 ± 0.7 µg/g, respectively. In addition, the endogenous folate vitamer 5-methlytetrahydrofolate (5-MTHF) was detected and quantified in SRM 1849a Infant/Adult Nutritional Formula, candidate SRM 1549a Whole Milk Powder, and candidate SRM 1845a Whole Egg Powder, resulting in values of 0.0839 ± 0.0071 µg/g, 0.211 ± 0.014 µg/g, and 0.838 ± 0.044 µg/g, respectively. The values obtained for folic acid and 5-MTHF by LC-MS/MS will be incorporated into the final value assignments in a variety of food-based SRMs.
Citation: Analytical and Bioanalytical Chemistry
Volume: 405
Pages: pp. 4561 - 4568
Keywords: folate; fortification; reference materials; LC-MS/MS
Research Areas: Organic Analytical Chemistry, Food and Nutrition, Standards, Measurements
DOI: http://dx.doi.org/10.1007/s00216-013-6733-4  (Note: May link to a non-U.S. Government webpage)