Take a sneak peek at the new NIST.gov and let us know what you think!
(Please note: some content may not be complete on the beta site.).
NIST Authors in Bold
|Author(s):||Daniel W. Bearden; Arezue F. Boroujerdi; Peter A. Lee; Giacomo R. DiTullio; Michael G. Janech; Sarah B. Vied;|
|Title:||Identification of Isethionic Acid and Other Small Molecule Metabolites of Fragilariopsis cylindrus with Nuclear Magnetic Resonance|
|Published:||July 08, 2012|
|Abstract:||Nuclear magnetic resonance (NMR)-spectroscopy has been used to obtain metabolic profiles of the polar diatom Fragilariopsis cylindrus, leading to the identification of a novel metabolite in this organism. Initial results from an ongoing metabolomics study have led to the discovery of isethionic acid (2-hydroxyethanesulphonic acid, CAS: 107-36-8) as a major metabolite in F. cylindrus. This compound is being produced by the organism under normal culture conditions. This finding is the first report of a diatom producing isethionic acid. In addition to isethionic acid, four other metabolites, dimethylsulfoniopropionate (DMSP), betaine, a trigonelline-like compound, and proline were present and may serve as osmoprotectants in F. cylindrus. NMR-based metabolite profiles of F. cylindrus were obtained along a growth curve of the organism. The relative concentration levels of the five metabolites were monitored over a growth period of F. cylindrus from 18 to 25 days. All showed an increase in relative concentration with time, except for proline, which began to decrease after day 21.|
|Citation:||Analytical and Bioanalytical Chemistry|
|Pages:||pp. 777 - 784|
|Keywords:||NMR, Isethionic Acid, diatom, Fragilariopsis cylindrus|
|Research Areas:||Climate Science Measurements, NMR, Marine Health|
|DOI:||http://dx.doi.org/10.1007/s00216-012-6169-2 (Note: May link to a non-U.S. Government webpage)|