Combinatorial Screening of Osteoblast Response to 3D Nano-Composite Tissue Scaffolds Using Gradients and Arrays

Published

April 24, 2011

Author(s)

Carl G. Simon Jr., Kaushik Chatterjee, Limin Sun, Laurence C. Chow, Marian F. Young

Abstract

Current methods for combinatorial and high-throughput (CHT) screening of cell-material interactions utilize a two-dimensional (2D) format where cells are presented on flat materials surfaces [1]. However, cell response in 3D scaffolds is more representative of their behavior in vivo [2]. Also, biomaterials must be fabricated into a 3D scaffold to generate a 3D tissue and cells are sensitive to topographical differences between 2D surfaces and 3D scaffolds. Thus, the objective in this work was to develop CHT methods to screen cell-material interactions in 3D. We used a bone tissue model to study osteoblast attachment and proliferation on porous poly(-caprolactone) (PCL) scaffolds containing amorphous calcium phosphate nanoparticles (nACP). nACP is highly soluble in water and releases calcium and phosphate ions that are known to enhance osteoblast response.

Conference Dates

April 13-16, 2011

Conference Location

Orlando, FL

Conference Title

Society for Biomaterials

Pub Type

Conferences

Keywords

polymer scaffold, osteoblast, cell adhesion, cell proliferation

Citation

Simon, C. , Chatterjee, K. , Sun, L. , Chow, L. and Young, M. (2011), Combinatorial Screening of Osteoblast Response to 3D Nano-Composite Tissue Scaffolds Using Gradients and Arrays, Society for Biomaterials, Orlando, FL (Accessed May 10, 2024)