| Author(s): |
Silvia H. De Paoli Lacerda; Jana Semberova; Karel Holada; Olga Simakova; Steven D. Hudson; Jan Simak; |
| Title: |
Carbon Nanotubes Activate Store Operated Calcium Entry (SOCE) in Blood Platelets Manifested by STIM-1 Clustering |
| Published: |
June 03, 2011 |
| Abstract: |
Intravascular effects of carbon nanotubes (CNTs) as a result of their various biomedical applications or environmental/occupational exposure raise serious safety concerns. CNTs potentiate arterial thrombosis in animal models;[1, 2] however, a mechanism of their prothrombotic effects has not been described. We have shown previously that different single-walled and multi-walled CNTs (MWCNTs), but not fullerene nC60, induce aggregation of blood platelets.[3][1] We found that MWCNTs activate blood platelets by inducing extracellular Ca2+ influx sensitive to calcium entry inhibitors. Here we show that MWCNTs penetrate through platelet membranes causing injury of the dense tubular system (DTS). This is associated with a marked decrease of calcium in the platelet intracellular stores. Moreover, we demonstrate that MWCNTs induce capping of STIM1 molecules, co-localized with Orai1 molecules in platelets, which indicates activation of the store-operated calcium entry (SOCE). Our findings elucidate a mechanism of the CNT-induced platelet activation, which is critical in blood biocompatibility of carbon nanomaterials. |
| Citation: |
Nature Nanotechnology |
| Volume: |
5 |
| Issue: |
7 |
| Pages: |
pp. 5808 - 5813 |
| Keywords: |
carbon nanotubes; blood platelet aggregation; calcium store; calcium release; nanoparticle; |
| Research Areas: |
Nanomaterials, Nanobiotechnology, Carbon Nanotubes |
| PDF version: |
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