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|Author(s):||Silvia H. De Paoli Lacerda; Jana Semberova; Karel Holada; Olga Simakova; Steven D. Hudson; Jan Simak;|
|Title:||Carbon Nanotubes Activate Store Operated Calcium Entry (SOCE) in Blood Platelets Manifested by STIM-1 Clustering|
|Published:||June 03, 2011|
|Abstract:||Intravascular effects of carbon nanotubes (CNTs) as a result of their various biomedical applications or environmental/occupational exposure raise serious safety concerns. CNTs potentiate arterial thrombosis in animal models;[1, 2] however, a mechanism of their prothrombotic effects has not been described. We have shown previously that different single-walled and multi-walled CNTs (MWCNTs), but not fullerene nC60, induce aggregation of blood platelets. We found that MWCNTs activate blood platelets by inducing extracellular Ca2+ influx sensitive to calcium entry inhibitors. Here we show that MWCNTs penetrate through platelet membranes causing injury of the dense tubular system (DTS). This is associated with a marked decrease of calcium in the platelet intracellular stores. Moreover, we demonstrate that MWCNTs induce capping of STIM1 molecules, co-localized with Orai1 molecules in platelets, which indicates activation of the store-operated calcium entry (SOCE). Our findings elucidate a mechanism of the CNT-induced platelet activation, which is critical in blood biocompatibility of carbon nanomaterials.|
|Pages:||pp. 5808 - 5813|
|Keywords:||carbon nanotubes, blood platelet aggregation, calcium store, calcium release, nanoparticle|
|Research Areas:||Nanomaterials, Nanobiotechnology, Carbon Nanotubes|
|PDF version:||Click here to retrieve PDF version of paper (932KB)|