Take a sneak peek at the new NIST.gov and let us know what you think!
(Please note: some content may not be complete on the beta site.).
NIST Authors in Bold
|Author(s):||Saugandhika Minnikanti; Darwin R. Reyes-Hernandez; Ruben C. Aguilar; Joseph Pancrazio; Michael Gaitan; Nathalia Peixoto;|
|Title:||Microfluidic Based Contactless DielectrophoreticDevice: Modeling and Analysis|
|Published:||September 01, 2010|
|Abstract:||While there have been many attempts at patterning cells onto substrates, a reliable method for trapping cell clusters and forming cell arrays in a predefined geometry remains to be demonstrated. We intend to develop a multielectrode array platform to initially trap cells via dielectrophoresis (DEP) and to later measure their electrical activity. As a first step toward that objective, here we present an interdigitated microfabricated comb structure. We designed an optimal insulation layer via finite element modeling for maximum dielectrophoretic field strength in solution and minimal cell damage. The microfabricated structure was combined with a microfluidic channel to vertically constrain cell position. With the objective of capturing cells onto the substrate, we here show that there is an optimal thickness of dielectric which limits electrolysis in solution and still allows for sufficient dielectrophoretic force on the cells to pull them onto the surface.|
|Research Areas:||Medical Devices, Life Sciences Research|
|PDF version:||Click here to retrieve PDF version of paper (897KB)|