NIST logo

Publication Citation: Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics

NIST Authors in Bold

Author(s): Joseph E. Reiner; John J. Kasianowicz; Joseph W. Robertson;
Title: Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics
Published: August 24, 2010
Abstract: Polyethylene glycol (PEG) is a ubiquitous charge-neutral polymer having several useful properties. It has been used to estimate the diameter of functioning nanopores. In this talk I will describe how the detailed analysis of PEG induced current blockades from a single alpha hemolysin channel in an unsupported lipid bilayer can be used to estimate step-wise changes in the structure of a functioning alpha hemolysin nanopore to < 1 Å. I will also describe a thermodynamic model that estimates the binding strength of cations to single PEG molecules from both the magnitude of PEG induced current blockades and the mean residence time of PEG within the nanopore.
Pages: 30 pp.
Research Areas: Nanobiotechnology, Single Molecule Biophysics