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Publication Citation: Development and Certification of a Standard Reference Material for Vitamin D Metabolites in Human Serum

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Author(s): Karen W. Phinney; Mary Bedner; Susan S. Tai; Veronica V. Vamathevan; Lane C. Sander; Katherine E. Sharpless; Stephen A. Wise;
Title: Development and Certification of a Standard Reference Material for Vitamin D Metabolites in Human Serum
Published: December 05, 2011
Abstract: The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health‰s Office of Dietary Supplements (NIH-ODS) has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D2, 25(OH)D3, and 3-epi-25(OH)D3. Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D3 proved to be essential for accurate determination of this metabolite.
Citation: Analytical Chemistry
Volume: 84
Pages: pp. 956 - 962
Keywords: epimer; isotope dilution; mass spectrometry; reference material; vitamin D
Research Areas: Standard Reference Materials, Mass Spectrometry, Organic Analytical Chemistry
DOI: http://dx.doi.org/10.1021/ac202047n  (Note: May link to a non-U.S. Government webpage)