| Author(s): |
Karen W. Phinney; Mary Bedner; Susan S. Tai; Veronica V. Vamathevan; Lane C. Sander; Katherine E. Sharpless; Stephen A. Wise; |
| Title: |
Development and Certification of a Standard Reference Material for Vitamin D Metabolites in Human Serum |
| Published: |
December 05, 2011 |
| Abstract: |
The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health’s Office of Dietary Supplements (NIH-ODS) has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D2, 25(OH)D3, and 3-epi-25(OH)D3. Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D3 proved to be essential for accurate determination of this metabolite. |
| Citation: |
Analytical Chemistry |
| Volume: |
84 |
| Pages: |
pp. 956 - 962 |
| Keywords: |
epimer; isotope dilution; mass spectrometry; reference material; vitamin D |
| Research Areas: |
Standard Reference Materials, Mass Spectrometry, Organic Analytical Chemistry |
| DOI: |
/10.1021/ac202047n (Note: May link to a non-U.S. Government webpage) |
