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|Author(s):||Darwin R. Reyes-Hernandez; Jennifer S. Hong; John T. Elliott; Michael Gaitan;|
|Title:||Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface|
|Published:||October 03, 2010|
|Abstract:||A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P19 cells is presented. A hybrid cell adhesive material (HCAM) comprised of a positively charged polyelectrolyte, polyallylamine hydrochloride (PAH), and fibronectin (FN), is deposited onto DEP electrodes to serve as an adhesive material for the DEP-trapped cells. Cells adhere onto the HCAM and under flow conditions, do not detach from the surface when DEP forces are removed.|
|Conference:||The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Proceedings:||Proceedings of Micro Total Analysis Systems 2010, Chemical and Biological Microsystems Society|
|Pages:||pp. 304 - 306|
|Dates:||October 3-7, 2010|
|Keywords:||dielectrophoresis, hybrid cell adhesive material, cell trapping,long-term cell assays|
|Research Areas:||Biomaterials, Bioscience & Health|
|PDF version:||Click here to retrieve PDF version of paper (2MB)|