| Author(s): |
Darwin R. Reyes-Hernandez; Jennifer S. Hong; John T. Elliott; Michael Gaitan; |
| Title: |
Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface |
| Published: |
October 03, 2010 |
| Abstract: |
A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P19 cells is presented. A hybrid cell adhesive material (HCAM) comprised of a positively charged polyelectrolyte, polyallylamine hydrochloride (PAH), and fibronectin (FN), is deposited onto DEP electrodes to serve as an adhesive material for the DEP-trapped cells. Cells adhere onto the HCAM and under flow conditions, do not detach from the surface when DEP forces are removed. |
| Conference: |
The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
|
| Proceedings: |
Proceedings of Micro Total Analysis Systems 2010, Chemical and Biological Microsystems Society |
| Pages: |
pp. 304 - 306 |
| Location: |
Groningen, -1 |
| Dates: |
October 3-7, 2010 |
| Keywords: |
dielectrophoresis; hybrid cell adhesive material; cell trapping;long-term cell assays |
| Research Areas: |
Biomaterials, Bioscience & Health |
| PDF version: |
 Click here to retrieve PDF version of paper (2MB) |
