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Publication Citation: Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface

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Author(s): Darwin R. Reyes-Hernandez; Jennifer S. Hong; John T. Elliott; Michael Gaitan;
Title: Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface
Published: October 03, 2010
Abstract: A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P19 cells is presented. A hybrid cell adhesive material (HCAM) comprised of a positively charged polyelectrolyte, polyallylamine hydrochloride (PAH), and fibronectin (FN), is deposited onto DEP electrodes to serve as an adhesive material for the DEP-trapped cells. Cells adhere onto the HCAM and under flow conditions, do not detach from the surface when DEP forces are removed.
Conference: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Proceedings: Proceedings of Micro Total Analysis Systems 2010, Chemical and Biological Microsystems Society
Pages: pp. 304 - 306
Location: Groningen, -1
Dates: October 3-7, 2010
Keywords: dielectrophoresis; hybrid cell adhesive material; cell trapping;long-term cell assays
Research Areas: Biomaterials, Bioscience & Health
PDF version: PDF Document Click here to retrieve PDF version of paper (2MB)