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Publication Citation: IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B

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Author(s): Atul Bhangale; Santanu S. Kundu; Wenchun Xie; William E. Wallace III; Kathleen M. Flynn; Kathryn L. Beers; Richard Gross;
Title: IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B
Published: January 01, 2010
Abstract: Epoxy functionalized supports are known to be suitable for industrial-scale immobilization of enzymes for biocatalysis. However, chemistry across these epoxy groups is known to affect the catalytic performance of the enzymes.In order to achieve a balance between immobilized enzyme activity and minimize enzyme leaching, immobilization supports with different physico-chemical properties were systematically studied. In the present study, we report immobilization of CAL B onto epoxy functionalized supports. CAL B was immobilized covalently on epoxy functionalized supports with different physico- chemical properties. Amongst them, Amberzyme and silanized silica support were selected as potential candidates and were studied in detail. In parallel, a microreactor based approach was developed in order to more systematically quantify the behavior of solid-supported catalysts in a continuous flow environment. Results indicates that covalently immobilized CAL B on silanized silica support showed better performance than that observed for methacrylate support, but lower biocatalytic performance as compared to physically immobilized CAL B on PMMA support.
Proceedings: American Chemical Society Spring 2010 National Meeting
Volume: 51
Issue: 1
Pages: pp. 760 - 761
Location: San Francisco, CA
Dates: March 21-25, 2010
Keywords: Microfluidics, CAL B, immobilization
Research Areas: Characterization, Combinatorial Methods, Microfluidics
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