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|Author(s):||Atul Bhangale; Santanu S. Kundu; Wenchun Xie; William E. Wallace; Kathleen M. Flynn; Kathryn L. Beers; Richard Gross;|
|Title:||IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B|
|Published:||January 01, 2010|
|Abstract:||Epoxy functionalized supports are known to be suitable for industrial-scale immobilization of enzymes for biocatalysis. However, chemistry across these epoxy groups is known to affect the catalytic performance of the enzymes.In order to achieve a balance between immobilized enzyme activity and minimize enzyme leaching, immobilization supports with different physico-chemical properties were systematically studied. In the present study, we report immobilization of CAL B onto epoxy functionalized supports. CAL B was immobilized covalently on epoxy functionalized supports with different physico- chemical properties. Amongst them, Amberzyme and silanized silica support were selected as potential candidates and were studied in detail. In parallel, a microreactor based approach was developed in order to more systematically quantify the behavior of solid-supported catalysts in a continuous flow environment. Results indicates that covalently immobilized CAL B on silanized silica support showed better performance than that observed for methacrylate support, but lower biocatalytic performance as compared to physically immobilized CAL B on PMMA support.|
|Proceedings:||American Chemical Society Spring 2010 National Meeting|
|Pages:||pp. 760 - 761|
|Location:||San Francisco, CA|
|Dates:||March 21-25, 2010|
|Keywords:||Microfluidics, CAL B, immobilization|
|Research Areas:||Characterization, Combinatorial Methods, Microfluidics|
|PDF version:||Click here to retrieve PDF version of paper (797KB)|