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|Author(s):||Joseph E. Reiner; John J. Kasianowicz; Brian J. Nablo; Joseph W. Robertson;|
|Title:||Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry|
|Published:||June 01, 2010|
|Abstract:||Nanometer-scale pores have demonstrated potential as a tool for the electrical detection and characterization of molecules. To provide a physical basis for nanopore-based analytical metrology, we show that a simple model quantitatively describes how one type of analyte (polymers of poly(ethylene glycol), PEG) reduces the ionic current in a single α-hemolysin ion channel. Two critical observations are apparent in the data. The depth of PEG-induced current blockades and the residence time of the polymer in the nanopore both decrease with increasing applied potential. A model is presented which describes these phenomena by analyzing the polymer size, and cation interactions with the polymer under moderate confinement.|
|Citation:||Proceedings of the National Academy of Sciences of the United States of America|
|Pages:||pp. 12080 - 12085|
|Keywords:||bioelectronics, nanopore sensing, single molecule analysis|
|PDF version:||Click here to retrieve PDF version of paper (1MB)|