| Author(s): |
Joseph E. Reiner; John J. Kasianowicz; Brian J. Nablo; Joseph W. Robertson; |
| Title: |
Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry |
| Published: |
June 01, 2010 |
| Abstract: |
Nanometer-scale pores have demonstrated potential as a tool for the electrical detection and characterization of molecules. To provide a physical basis for nanopore-based analytical metrology, we show that a simple model quantitatively describes how one type of analyte (polymers of poly(ethylene glycol), PEG) reduces the ionic current in a single α-hemolysin ion channel. Two critical observations are apparent in the data. The depth of PEG-induced current blockades and the residence time of the polymer in the nanopore both decrease with increasing applied potential. A model is presented which describes these phenomena by analyzing the polymer size, and cation interactions with the polymer under moderate confinement. |
| Citation: |
Proceedings of the National Academy of Sciences of the United States of America |
| Volume: |
107 |
| Pages: |
pp. 12080 - 12085 |
| Keywords: |
bioelectronics; nanopore sensing; single molecule analysis |
| Research Areas: |
Nanobiotechnology |
| PDF version: |
 Click here to retrieve PDF version of paper (1MB) |
