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Publication Citation: Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry

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Author(s): Joseph E. Reiner; John J. Kasianowicz; Brian J. Nablo; Joseph W. Robertson;
Title: Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry
Published: June 01, 2010
Abstract: Nanometer-scale pores have demonstrated potential as a tool for the electrical detection and characterization of molecules. To provide a physical basis for nanopore-based analytical metrology, we show that a simple model quantitatively describes how one type of analyte (polymers of poly(ethylene glycol), PEG) reduces the ionic current in a single α-hemolysin ion channel. Two critical observations are apparent in the data. The depth of PEG-induced current blockades and the residence time of the polymer in the nanopore both decrease with increasing applied potential. A model is presented which describes these phenomena by analyzing the polymer size, and cation interactions with the polymer under moderate confinement.
Citation: Proceedings of the National Academy of Sciences of the United States of America
Volume: 107
Pages: pp. 12080 - 12085
Keywords: bioelectronics; nanopore sensing; single molecule analysis
Research Areas: Nanobiotechnology
PDF version: PDF Document Click here to retrieve PDF version of paper (1MB)